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ospC Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS505074

The Borrelia burgdorferi antigen ospC has been prepared as a recomhinant antigen fused to a his-tag in its N-terminus. More details

$1195 / 1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ospC, Recombinant Protein
Full Product Name
Recombinant antigen ospC for Borrelia
Product Gene Name
ospC recombinant protein
Product Synonym Names
B. burgdorferi
Host
Escherichia coli
Form/Format
20 mM phosphate buffer pH 7 and 0.15 M NaCl (Before lyophilization)
Purity/Purification
>95% in a SDS-PAGE
Concentration
1.91 mg/ml (lot specific)
Preparation and Storage
Protein is shipped at room temperature. Upon arrival, it should be stored at 4°C or -20°C in vertical position, avoiding all possible humidity and maintaining the vials dry. Once reconstituted, it should be aliquoted in order to avoid repeated freezing and thawing cycles and stored at -20°C to -80°C.
Molecular Weight
Determined by SDS-PAGE, the protein band is between molecular markers of 25,000 and 35,000 Da, while relative molecular mass calculated from amino acid sequence is 27,887.3 Da.
Recombinant Antigen
The outer surface protein C (ospC) of Borrelia burgdorferi (Lagal et al., 2003).
Batch Composition
Components: his-ospC
Compostition: recombinant antigen with a his-tag in its N-terminus.
Total Quality Per Aliquot
1 mg
Observations
Proteins should be maintained frozen at high concentrations. The dllution to be performed for ELISA assays should be made with a small quantity of protein, the same day of the experiment. In order to defrost the protein, maintain the aliquot at 25°C without shaking to avoid aggregation. Prior making test dilutions and after defrosting the protein, is recommended to remove possible protein aggregates by centrifuging the stock solution, avoiding alterations in the immobilization of the biomolecule to the solid surface.
Reconstitution
With approximately 0.66 ml of sterile double-distilled water, a final concentration of approximately 1.3 mg/ml will be obtianed. The solubilisation of the cake should be developed for 15 mnutes to allow a homogenous protein solution, considering that part ofthe cake can be on the glass-walls of the container. With this reconstitution, the protein will be maintained at pH 7. It is recommended that the users carry out their absorbance determinations to avoid concentration variabilities due to the equipment used, mainly in reproducibility analysis.

Product Categories/Family for ospC recombinant protein

BACTERIA
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Application Notes for ospC recombinant protein
Absence of precipitation after a freezing thawing cycle: Ok
Possible applications : Western Blot (WB), Dot Blot (DB), Indirect ELISA, Positive Control in direct ELISA, CLIA, Lateral Flow.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates.

Quality Control of ospC recombinant protein

This protein does not contain any Trp residues. Experience shows that this could result in more than 10% error in the computed extinction coefficient. Therefore, we have measured the protein concentration by using the colorimetric assay based on the interaction between Coomassie brilliant blue and the arginine and aromatic residues (Bradford Method) and its maximum absorption shifts from 470 nm to 595 nm. The standard curve was performed with the protein BSA. 40 ul of the protein were analysed.
ospC Recombinant Protein – Cat# MBS505074

Purity Control in SDS-PAGE of ospC recombinant protein

SDS-PAGE analysis (15%) of 2 ul of recombinant ospC. Purity is > 95% as determined by gel electrophoresis. Bands which slightly appear under the main band correspond to this same protein as it is showed in a western blot performed with a his-tag monoclonal antibody.
ospC Recombinant Protein, Purity Control in SDS-PAGE – Cat# MBS505074

Other Notes

Small volumes of ospC recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ospC purchase

MBS505074 is a recombinant protein targeting ospC. Also known as Recombinant antigen ospC for Borrelia. Host: Escherichia coli.

Additional Details

NCBI/Uniprot data below describe general gene information for ospC. It may not necessarily be applicable to this product.

NCBI GI #
444898153
NCBI Accession #
AGE13739.1
NCBI Official Full Name
OspC, partial
UniProt Protein Name
OspC
UniProt Synonym Protein Names
OspC
Protein Family
Outer surface protein
UniProt Gene Name
ospC

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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