• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

PHL p 5b Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS505081

Recombinant Allergen: Phleum pratense Phl p 5 is one of major pollen allergen of timothy grass and accounts for IgE binding in up to 60% of patients (Vrtala et al., 1993). Phl p 5b is one of the isoallergenic groups of Phl p 5 (van Neerven et al.,… More details

$1195 / 1 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Phl p 5b, Recombinant Protein
Full Product Name
Recombinant allergen Phl p 5b for Phleum pratense (Timothy grass pollen)
Product Gene Name
Phl p 5b recombinant protein
Host
E Coli
Form/Format
Dry powder (lyophilized)
Purity/Purification
15% SDS-PAGE
Concentration
1.44 mg/ml (lot specific)
Preparation and Storage
Lyophilized protein is shipped with on ice packs. Upon arrival, it should be stored at 20 degree C to -80 degree C. Aliquot after reconstitution in order to avoid repeated freezing and thawing cycles and store at 20 degree C to -80 degree C.
Molecular Weight
Predicted MW: 32.7 kDa
Observed MW: 25-35 kDa
Organism
Plehum Pratense
Storage Buffer
50 mM MES pH 6.5 and 0.1 M KCl
Quality Control
1. Protein concentration determined spectrophotometrically.
2. Purity control in SDS-PAGE: 15%
3. No precipitate observed after a freezing and thawing cycle.
Reconstitution
For lyophilized proteins: with 0.04 ml of sterile double-distilled water (lot specific) for a final concentration of 1.59 mg/ml (lot specific). The protein will be at pH 8.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for Phl p 5b recombinant protein
ELISA (EIA)
Application Notes for Phl p 5b recombinant protein
The evaluation of the recombinant allergen has been performed by means of an in-house ELISA assay performed in a spanish hospital. This immunoassay was carried out with a serum sample panel of 25 positive and 10 negative specimen sera, pre-validated by skin prick testing (SPT) and the UniCAP test.
The recombinant allergen Phl p 5b detected 14 positive sera out of 25 (56% incidence) with higher prevalence of sera with titers of 3.5-17.5 IU/ml (international units per milliliter; 1 IU is equivalent to 2.42 ng of IgE).

SDS-Page of Phl p 5b recombinant protein

Figure 1. SDS-PAGE analysis (15%) of 4 ul of recombinant allergen. Purity is > 95% as determined by gel electrophoresis.
PHL p 5b Recombinant Protein – Cat# MBS505081

Testing Data of Phl p 5b recombinant protein

This protein does not contain any Trp residues. Experience shows that this could result in more than 10% error in the computed extinction coefficient. Therefore, we have meassured the protein concentration by using the colorimetric assay based on the interaction between Coomassie brilliant blue and the arginine and aromatic residues (Bradford Method) and its maximum absorption shifts from 470 nm to 595 nm. The standard curve was performed with the protein BSA. 40 ul of the protein were analysed.
DO595 = 0.746
PHL p 5b Recombinant Protein, Testing Data – Cat# MBS505081

Product References and Citations for Phl p 5b recombinant protein

Vrtala, S., Sperr, W.R., Reimitzer, I., van Ree, R., Laffer, S., Muller, W.D., Valent, P., Lechner, K.,Rumpold, H., Kraft, D. cDNA cloning of a major allergen from timothy grass (Phleum pratense) pollen; characterization of the recombinant Phl pV allergen. 1993. J. Immunol. 151, 4773-4781.
van Neerven RJ1, Wikborg T, Lund G, Jacobsen B, Brinch-Nielsen A, Arnved J, Ipsen H. Blocking antibodies induced by specific allergy vaccination prevent the activation of CD4+ T cells by inhibiting serum-IgEfacilitated allergen presentation. 1999. J Immunol. 163:2944-52.
Bradford, MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem. 1976, 131:499-503.

Other Notes

Small volumes of Phl p 5b recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Phl p 5b purchase

MBS505081 is a recombinant protein targeting Phl p 5b. Also known as Recombinant allergen Phl p 5b for Phleum pratense (Timothy grass pollen). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for Phl p 5b. It may not necessarily be applicable to this product.

Protein Family
Pollen allergen

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000