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OLE e 1 Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS505084

Recombinant Allergen: Olea europaea ole e 1 olive tree allergen (Asturias et al., 1997).Description: Ole e 1 is the most prevalent allergen of olive tree pollen. This allergen has been prepared as a recombinant mature protein fused to a his-tag in its C… More details

$1335 / 1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Ole e 1, Recombinant Protein
Full Product Name
Recombinant allergen Ole e 1 for Olea europaea (olive tree)
Product Gene Name
Ole e 1 recombinant protein
Host
Pichia pastoris
Form/Format
Dry powder (lyophilized)
Purity/Purification
15% SDS-PAGE
Concentration
1.20 mg/ml (lot specific)
Preparation and Storage
Lyophilized protein is shipped with on ice packs. Upon arrival, it should be stored at 20 degree C to -80 degree C. Aliquot after reconstitution in order to avoid repeated freezing and thawing cycles and store at 20 degree C to -80 degree C.
Molecular Weight
Predicted MW: 18.8 kDa
Observed MW: 25-35 kDa
Organism
Olea Europaea
Storage Buffer
20 mM phosphate buffer pH 8 and 0.15 M NaCl
Quality Control
1. Protein concentration determined spectrophotometrically.
2. Purity control in SDS-PAGE: 15%
3. No precipitate observed after a freezing and thawing cycle.
Protein Fingerprint by Mass Spectrometry
Top Score: 117 for ole e 1
Protein score is -10*Log(P), where P is the probability that the observed match is a random event. Protein scores greater than 13 are significant (p<0.05).
The MS was performed with a by MALDI TOF/TOF model UltrafleXtreme (Bruker).
Reconstitution
For lyophilized proteins: with 0.04 ml of sterile double-distilled water (lot specific) for a final concentration of 1.59 mg/ml (lot specific). The protein will be at pH 8.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Application Notes for Ole e 1 recombinant protein
This biomarker has been evaluated in an external study carried out at a Spanish hospital by a group of allergists with positive and negative serum samples from patients. The evaluation of the recombinant allergens has been performed by means of an in-house ELISA assay. In this immunoassay, it has been determined the presence of specific IgE in sera that had previously been validated by skin prick testing (SPT) and the UniCAP test. The sera panel for this study was composed of 25 positive and 10 negative specimen sera.
The recombinant allergen ole e 1 detected 25 positive sera out of 25 (100% incidence) with higher prevalence of sera with titers of 3.5-17.50 IU/ml (international units per milliliter; 1 IU is equivalent to 2.42 ng of IgE).

SDS-Page of Ole e 1 recombinant protein

Figure 1. SDS-PAGE analysis (15%) of 8 ul of the recombinant allergen ole e 1. Purity is >95% as determined by gel electrophoresis.
OLE e 1 Recombinant Protein – Cat# MBS505084

Testing Data of Ole e 1 recombinant protein

OLE e 1 Recombinant Protein, Testing Data – Cat# MBS505084

Product References and Citations for Ole e 1 recombinant protein

Asturias, JA et al. Cloning and expression of the panallergen profiling and the major allergen (Ole e 1) from olive tree pollen. 1997, J. Allergy Clin Immunol 100:365-72.
Gill SC, von Hippel PH. Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem. 1989 Nov 1;182(2):319-26.

Other Notes

Small volumes of Ole e 1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Ole e 1 purchase

MBS505084 is a recombinant protein targeting Ole e 1. Also known as Recombinant allergen Ole e 1 for Olea europaea (olive tree). Host: Pichia pastoris.

Additional Details

NCBI/Uniprot data below describe general gene information for Ole e 1. It may not necessarily be applicable to this product.

Protein Family
Major pollen allergen

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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