p33ING2 Recombinant Protein

Function: Seems to be involved in p53/TP53 activation and p53/TP53-dependent apoptotic pathways, probably by enhancing acetylation of p53/TP53. Component of a mSin3A-like corepressor complex, which is probably involved in deacetylation of nucleosomal histones. ING2 activity seems to be modulated by binding to phosphoinositides (PtdInsPs). Ref.2 Ref.9

Subunit structure: Interacts with H3K4me3 and to a lesser extent with H3K4me2. Component of a mSin3A-like complex at least consisting of SIN3A, HDAC1, HDAC2, RBBP4/RbAp48, RBBP7/RbAp46, SAP30 and ING2. Ref.8 Ref.11 Ref.12

Subcellular location: Nucleus. Note: Predominantly nuclear. Localized to chromatin and nuclear matrix. Upon reduced PtdIns5P levels seems to be released from chromatin and, at least partially, translocated to the cytoplasm. Ref.9

Tissue specificity: Widely expressed. Higher expressed in colon-cancer tumor than in normal colon tissues. Ref.1

Induction: Induced by the DNA-damaging agents etoposide and neocarzinostatin. Ref.2

Domain: The PHD-type zinc finger mediates the binding to H3K4me3. Ref.12The polybasic region (PBR) is responsive to the binding to phosphoinositides (PtdInsPs), including phosphatidylinositol 5-phosphate (PtdIns5P). Ref.12

Post-translational modification: Sumoylation enhances its association with SIN3A and is required for binding to some target gene promoters, this is the case for TMEM71. Ref.13

Sequence similarities: Belongs to the ING family.Contains 1 PHD-type zinc finger.