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Phospholipase B (PLB) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2027529
SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment

MBS2027529 is an antibody targeting PLB. Also known as Polyclonal Antibody to Phospholipase B (PLB). Reactive in Human. Host: Rabbit. More details

$215 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Phospholipase B (PLB), Polyclonal Antibody
Full Product Name
Polyclonal Antibody to Phospholipase B (PLB)
Product Gene Name
anti-PLB antibody
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Matching Pairs
Unconjugated Antibody: PLB (MBS2027529)
Immunogen: PLB (MBS2030040)
Matching Pairs
Unconjugated Antibody: PLB (MBS2027529)
APC-CY7 Conjugated Antibody: PLB (MBS2062518)
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Molecular Weight
47,341 Da
OMIM
AC074011 Genomic DNA
3D Structure
ModBase 3D Structure for Q6P1J6
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Organism Species
Homo sapiens
Source
Polyclonal antibody preparation
Traits
Liquid
Immunogen
Recombinant PLB (Met1036~Leu1458) expressed in E Coli.
Cross Reactivity
Human
Conjugate
No Conjugate
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2062520
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-PLB antibody
WB; IHC; ICC; IP.
Application Notes for anti-PLB antibody
Western blotting: 0.5-2 ug/mL;1:500-2000
Immunohistochemistry: 5-20 ug/mL;1:50-200
Immunocytochemistry: 5-20 ug/mL;1:50-200

Optimal working dilutions must be determined by end user.

Western Blot (WB) of anti-PLB antibody

Western Blot: Sample: Human Serum; Primary Ab: 2ug/ml Rabbit Anti-Human PLB Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
Rabbit Anti-PLB Polyclonal Antibody – Cat# MBS2027529

Western Blot (WB) of anti-PLB antibody

Western Blot: Sample: Recombinant protein.
Rabbit Anti-PLB Polyclonal Antibody, Western Blot (WB) – Cat# MBS2027529

Immunohistochemistry (IHC) of anti-PLB antibody

DAB staining on IHC-P. Samples: Human Tissue)
Rabbit Anti-PLB Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2027529

Other Notes

Small volumes of anti-PLB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PLB. It may not necessarily be applicable to this product.

NCBI GI #
283436114
NCBI GeneID
151056
NCBI Accession #
NP_001164056.1
UniProt Primary Accession #
Q6P1J6
UniProt Secondary Accession #
Q53S03; Q8IUP7; Q96DP9; A8KAX2
UniProt Related Accession #
Q6P1J6
GenBank Nucleotide #
NM_001170585.1
NCBI Official Full Name
phospholipase B1, membrane-associated isoform 2
NCBI Official Synonym Full Names
phospholipase B1
NCBI Official Symbol
PLB1
NCBI Official Synonym Symbols
PLB; PLB/LIP
UniProt Protein Name
Phospholipase B1, membrane-associated
UniProt Synonym Protein Names
Phospholipase B/lipase; PLB/LIP
Protein Family
Lysophospholipase
UniProt Gene Name
PLB1
UniProt Synonym Gene Names
PLB; Phospholipase B; hPLB; PLB/LIP
NCBI Protein Information
phospholipase B1, membrane-associated

NCBI Summary for PLB

This gene encodes a membrane-associated phospholipase that displays lysophospholipase and phospholipase A2 activities through removal of sn-1 and sn-2 fatty acids of glycerophospholipids. In addition, it displays lipase and retinyl ester hydrolase activities. The encoded protein is highly conserved and is composed of a large, glycosylated extracellular domain composed of four tandem homologous domains, followed by a hydrophobic segment that anchors the enzyme to the membrane and a short C-terminal cytoplasmic tail. This gene has been identified as a candidate rheumatoid arthritis risk gene. [provided by RefSeq, Jul 2016]
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UniProt Comments for PLB

PLB1: Membrane-associated phospholipase. Exhibits a calcium- independent broad substrate specificity including phospholipase A2/lysophospholipase activity. Preferential hydrolysis at the sn-2 position of diacylphospholipids and diacyglycerol, whereas it shows no positional specificity toward triacylglycerol. Exhibits also esterase activity toward p-nitrophenyl. May act on the brush border membrane to facilitate the absorption of digested lipids. Belongs to the 'GDSL' lipolytic enzyme family. Phospholipase B1 subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.1.4; EC 3.1.1.5; Membrane protein, integral; Phospholipase

Chromosomal Location of Human Ortholog: 2p23.2

Cellular Component: apical plasma membrane; integral component of membrane; plasma membrane

Molecular Function: lysophospholipase activity; phospholipase A2 activity; retinyl-palmitate esterase activity

Biological Process: lipid catabolic process; retinoid metabolic process; retinol metabolic process
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Research Articles on PLB

1. rs117512489 in gene phospholipase B1 gene is associated with Non-Small Cell Lung Cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PLB antibody

 Reference Product  PubMed Publications
 PLA2G4A antibody  >2 publications with PLB and PLA2G4A
 PLD1 antibody  >1 publications with PLB and PLD1

Pathways associated with anti-PLB antibody

 Products by Pathway  Pathway Diagram
 Acyl Chain Remodelling Of PC Pathway antibodies  Acyl Chain Remodelling Of PC Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Ether Lipid Metabolism Pathway antibodies  Ether Lipid Metabolism Pathway Diagram
 Ether Lipid Metabolism Pathway antibodies  Ether Lipid Metabolism Pathway Diagram
 Glycerophospholipid Biosynthesis Pathway antibodies  Glycerophospholipid Biosynthesis Pathway Diagram
 Glycerophospholipid Metabolism Pathway antibodies  Glycerophospholipid Metabolism Pathway Diagram
 Glycerophospholipid Metabolism Pathway antibodies  Glycerophospholipid Metabolism Pathway Diagram
 Linoleic Acid Metabolism Pathway antibodies  Linoleic Acid Metabolism Pathway Diagram
 Linoleic Acid Metabolism Pathway antibodies  Linoleic Acid Metabolism Pathway Diagram

Diseases associated with anti-PLB antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >3 publications with PLB and Inflammation
 Breast Neoplasms Antibodies  >1 publications with PLB and Breast Neoplasms
 Neoplasms, Experimental Antibodies  >1 publications with PLB and Neoplasms, Experimental
 Carcinoma Antibodies  >1 publications with PLB and Carcinoma
 Necrosis Antibodies  >1 publications with PLB and Necrosis
 Arthritis, Rheumatoid Antibodies  >1 publications with PLB and Arthritis, Rheumatoid

Organs/Tissues associated with anti-PLB antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >8 publications with PLB and Blood
 Brain Antibodies  >7 publications with PLB and Brain
 Lung Antibodies  >6 publications with PLB and Lung
 Kidney Antibodies  >1 publications with PLB and Kidney
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