GAB1 Recombinant Protein

Name: GAB1 Recombinant Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS515364
Price: 350 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS515364

Recombinant full-length human GAB1 was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.Scientific Background: GAB1 is a member of the IRS1-like multisubstrate docking protein family and is a direct substrate of the epidermal growth… More details

$350 / 0.02 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

GAB1, Recombinant Protein

Full Product Name

GAB1 recombinant protein

Product Gene Name

GAB1 recombinant protein

Product Synonym Gene Name

none

Host

Sf9 insect cells

Form/Format

50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.

Preparation and Storage

Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Molecular Weight

~108 kDa

Chromosome Location

Chromosome: 4; NC_000004.11 (144257680..144395718). Location: 4q31.21

OMIM

604439

3D Structure

ModBase 3D Structure for Q13480

Type

Recombinant Fusion Protein

Species

Human

Tag Information

His tag

Expression System

Sf9 insect cells using baculovirus

Source Note

Recombinant full-length human GAB1 was expressed by baculovirus in Sf9 insect cells

Dry Ice Shipment

Extra charge fee may add to your shipping cost as dry ice is required to ship this product.

Product Categories/Family for GAB1 recombinant protein

Adaptor Proteins; Signaling Proteins - Adaptor Proteins

Applications Tested/Suitable for GAB1 recombinant protein

Western Blot (WB)

Product References and Citations for GAB1 recombinant protein

1.Holgado-Madruga M, et al: A Grb2-associated docking protein in EGF- and insulin-receptor signalling. Nature 379: 560-564, 1996.
2.Kiyatkin A, et al: Scaffolding protein Grb2-associated binder 1 sustains epidermal growth factor-induced mitogenic and survival signaling by multiple positive feedback loops. J Biol Chem. 2006 Jul 21;281(29):19925-38.

Other Notes

Small volumes of GAB1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GAB1 purchase

MBS515364 is a recombinant protein targeting GAB1. Host: Sf9 insect cells.

Additional Details

NCBI/Uniprot data below describe general gene information for GAB1. It may not necessarily be applicable to this product.

NCBI GI #

195927064

NCBI GeneID

2549

NCBI Accession #

NP_997006.1

UniProt Primary Accession #

Q13480

UniProt Secondary Accession #

Q4W5G2; Q6P1W2; A8K152

UniProt Related Accession #

Q13480

GenBank Nucleotide #

NM_207123

NCBI Official Full Name

Homo sapiens GRB2-associated binding protein 1 (GAB1), transcript variant 1, mRNA

NCBI Official Synonym Full Names

GRB2-associated binding protein 1

NCBI Official Symbol

GAB1

UniProt Protein Name

GRB2-associated-binding protein 1

UniProt Synonym Protein Names

GRB2-associated binder 1; Growth factor receptor bound protein 2-associated protein 1

Protein Family

GPI transamidase component

UniProt Gene Name

GAB1

UniProt Entry Name

GAB1_HUMAN

NCBI Protein Information

GRB2-associated-binding protein 1; GRB2-associated binder 1; growth factor receptor bound protein 2-associated protein 1

NCBI Summary for GAB1

The protein encoded by this gene is a member of the IRS1-like multisubstrate docking protein family. It is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

UniProt Comments for GAB1

Function: Adapter protein that plays a role in intracellular signaling cascades triggered by activated receptor-type kinases. Plays a role in FGFR1 signaling. Probably involved in signaling by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR).

Subunit structure: Interacts with GRB2 and with other SH2-containing proteins. Interacts with phosphorylated LAT2. Interacts with PTPRJ. Identified in a complex containing FRS2, GRB2, GAB1, PIK3R1 and SOS1. Interacts (phosphorylated) with PTPN11. Interacts with HCK. Ref.7 Ref.8 Ref.9

Post-translational modification: Phosphorylated in response to FGFR1 activation. Phosphorylated on tyrosine residue(s) by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Tyrosine phosphorylation of GAB1 mediates interaction with several proteins that contain SH2 domains. Phosphorylated on tyrosine residues by HCK upon IL6 signaling. Ref.9

Sequence similarities: Belongs to the GAB family.Contains 1 PH domain.

Research Articles on GAB1

1. the acquired substrate preference for GAB1 is critical for the ERBB2 mutant-induced oncogenesis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GAB1 recombinant protein

Reference Product	PubMed Publications
GRB2 recombinant protein	>184 publications with GAB1 and GRB2
MET recombinant protein	>91 publications with GAB1 and MET
PTPN11 recombinant protein	>86 publications with GAB1 and PTPN11
EGFR recombinant protein	>48 publications with GAB1 and EGFR
CRK recombinant protein	>21 publications with GAB1 and CRK
SHC1 recombinant protein	>9 publications with GAB1 and SHC1
CRKL recombinant protein	>6 publications with GAB1 and CRKL
GRAP2 recombinant protein	>2 publications with GAB1 and GRAP2
PIK3R1 recombinant protein	>1 publications with GAB1 and PIK3R1

Pathways associated with GAB1 recombinant protein

Products by Pathway	Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
B Cell Receptor Signaling Pathway antibodies	B Cell Receptor Signaling Pathway Diagram
Bacterial Invasion Of Epithelial Cells Pathway antibodies	Bacterial Invasion Of Epithelial Cells Pathway Diagram
Bacterial Invasion Of Epithelial Cells Pathway antibodies	Bacterial Invasion Of Epithelial Cells Pathway Diagram
Constitutive PI3K/AKT Signaling In Cancer Pathway antibodies	Constitutive PI3K/AKT Signaling In Cancer Pathway Diagram
DAP12 Interactions Pathway antibodies	DAP12 Interactions Pathway Diagram
DAP12 Signaling Pathway antibodies	DAP12 Signaling Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
Downstream Signaling Events Of B Cell Receptor (BCR) Pathway antibodies	Downstream Signaling Events Of B Cell Receptor (BCR) Pathway Diagram
Downstream Signal Transduction Pathway antibodies	Downstream Signal Transduction Pathway Diagram

Diseases associated with GAB1 recombinant protein

Disease Name	Pubmed Publications
Neoplasms Antibodies	>91 publications with GAB1 and Neoplasms
Breast Neoplasms Antibodies	>11 publications with GAB1 and Breast Neoplasms
Disease Models, Animal Antibodies	>9 publications with GAB1 and Disease Models, Animal
Neoplasms, Experimental Antibodies	>9 publications with GAB1 and Neoplasms, Experimental
Inflammation Antibodies	>9 publications with GAB1 and Inflammation
Necrosis Antibodies	>7 publications with GAB1 and Necrosis
Nervous System Diseases Antibodies	>7 publications with GAB1 and Nervous System Diseases
Adenocarcinoma Antibodies	>6 publications with GAB1 and Adenocarcinoma
Insulin Resistance Antibodies	>6 publications with GAB1 and Insulin Resistance
Atherosclerosis Antibodies	>5 publications with GAB1 and Atherosclerosis

Organs/Tissues associated with GAB1 recombinant protein

Organ/Tissue Name	Pubmed Publications
Vascular Antibodies	>30 publications with GAB1 and Vascular
Muscle Antibodies	>28 publications with GAB1 and Muscle
Kidney Antibodies	>16 publications with GAB1 and Kidney
Lung Antibodies	>16 publications with GAB1 and Lung
Embryonic Tissue Antibodies	>16 publications with GAB1 and Embryonic Tissue
Nerve Antibodies	>16 publications with GAB1 and Nerve
Brain Antibodies	>15 publications with GAB1 and Brain
Liver Antibodies	>12 publications with GAB1 and Liver
Heart Antibodies	>10 publications with GAB1 and Heart
Placenta Antibodies	>10 publications with GAB1 and Placenta