TOPK Recombinant Protein

This gene encodes a serine/threonine protein kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. The encoded protein may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. Overexpression of this gene has been implicated in tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Function: Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage. Ref.1 Ref.7

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation: Activated by phosphorylation

By similarity.

Subunit structure: Interacts with DLG1 and TP53. Ref.2 Ref.7

Tissue specificity: Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules. Ref.1 Ref.3

Post-translational modification: Phosphorylated; in a cell-cycle dependent manner at mitosis. Ref.1 Ref.2

Sequence similarities: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.Contains 1 protein kinase domain.