SETDB2 Recombinant Protein

Name: SETDB2 Recombinant Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS515393
Price: 350 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS515393

Recombinant full-length human SETDB2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.Scientific Background: SETDB2 is a histone H3 methyltransferase that modulates gene expression epigenetically through histone H3 methylation.… More details

$350 / 0.02 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

SETDB2, Recombinant Protein

Full Product Name

SETDB2 recombinant protein

Product Gene Name

SETDB2 recombinant protein

Product Synonym Gene Name

CLLD8; CLLL8

Product Synonym Names

CLLD8; CLLL8

Host

Sf9 insect cells

Form/Format

50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Preparation and Storage

Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Molecular Weight

~120 kDa

Chromosome Location

Chromosome: 13; NC_000013.10 (50018429..50069139). Location: 13q14

OMIM

607865

3D Structure

ModBase 3D Structure for Q96T68

Type

Recombinant Fusion Protein

Species

Human

Tag Information

GST tag

Expression System

Sf9 insect cells using baculovirus

Source Note

Recombinant full-length human SETDB2 was expressed by baculovirus in Sf9 insect cells

Dry Ice Shipment

Extra charge fee may add to your shipping cost as dry ice is required to ship this product.

Product Categories/Family for SETDB2 recombinant protein

Acetyl/Methyltransferases Proteins; Signaling Proteins - Acetyl/Methyltransferase Proteins

Applications Tested/Suitable for SETDB2 recombinant protein

Western Blot (WB)

Product References and Citations for SETDB2 recombinant protein

1. Falandry C. et al: CLLD8/KMT1F is a lysine methyltransferase that is important for chromosome segregation. J Biol Chem. 2010 Jun 25;285(26):20234-41.
2. Zhang, Y. et al: Positional cloning of a quantitative trait locus on chromosome 13q14 that influences immunoglobulin E levels and asthma. Nature Genet. 34: 181-186, 2003.

Other Notes

Small volumes of SETDB2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SETDB2 purchase

MBS515393 is a recombinant protein targeting SETDB2. Host: Sf9 insect cells.

Additional Details

NCBI/Uniprot data below describe general gene information for SETDB2. It may not necessarily be applicable to this product.

NCBI GI #

34193430

NCBI GeneID

83852

NCBI Accession #

BC047434

UniProt Primary Accession #

Q96T68

UniProt Secondary Accession #

Q5TC65; Q5TC66; Q5W0A7; Q659A7; Q86UD6; Q96AI6

UniProt Related Accession #

Q96T68

NCBI Official Full Name

Homo sapiens SET domain, bifurcated 2, mRNA

NCBI Official Synonym Full Names

SET domain, bifurcated 2

NCBI Official Symbol

SETDB2

NCBI Official Synonym Symbols

CLLD8; CLLL8; KMT1F; C13orf4

UniProt Protein Name

Histone-lysine N-methyltransferase SETDB2

UniProt Synonym Protein Names

Chronic lymphocytic leukemia deletion region gene 8 protein; Lysine N-methyltransferase 1F; SET domain bifurcated 2

Protein Family

Histone-lysine N-methyltransferase

UniProt Gene Name

SETDB2

UniProt Synonym Gene Names

C13orf4; CLLD8; KMT1F

UniProt Entry Name

SETB2_HUMAN

NCBI Protein Information

histone-lysine N-methyltransferase SETDB2; lysine N-methyltransferase 1F; chronic lymphocytic leukemia deletion region 8; chronic lymphocytic leukemia deletion region gene 8 protein

NCBI Summary for SETDB2

Proteins that contain a SET domain, such as SETDB2, modulate gene expression epigenetically through histone H3 (see MIM 601128) methylation. SETDB2 is likely a histone H3 methyltransferase, as it contains both the active site and flanking cysteine residues required for catalytic activity (Zhang et al., 2003 [PubMed 12754510]).[supplied by OMIM, Mar 2008]

UniProt Comments for SETDB2

Function: Histone methyltransferase involved in left-right axis specification in early development and mitosis. Specifically trimethylates 'Lys-9' of histone H3 (H3K9me3). H3K9me3 is a specific tag for epigenetic transcriptional repression that recruits HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Contributes to H3K9me3 in both the interspersed repetitive elements and centromere-associated repeats. Plays a role in chromosome condensation and segregation during mitosis. Ref.5

Catalytic activity: S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].

Subcellular location: Nucleus. Chromosome

Probable Ref.5.

Tissue specificity: Ubiquitous. Highest expression in heart, testis and ovary.

Sequence similarities: Belongs to the class V-like SAM-binding methyltransferase superfamily.Contains 1 MBD (methyl-CpG-binding) domain.Contains 1 pre-SET domain.Contains 1 SET domain.

Sequence caution: The sequence CAH56265.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.The sequence CAI10818.2 differs from that shown. Reason: Erroneous gene model prediction.

Research Articles on SETDB2

1. Results provide evidence that CLLD8/KMT1F is recruited to heterochromatin regions and contributes in vivo to the deposition of trimethyl marks in concert with SUV39H1/KMT1A.SUV39H1/KMT1A.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with SETDB2 recombinant protein

Reference Product	PubMed Publications
PHF11 recombinant protein	>4 publications with SETDB2 and PHF11
RCBTB1 recombinant protein	>2 publications with SETDB2 and RCBTB1
MBD6 recombinant protein	>1 publications with SETDB2 and MBD6

Pathways associated with SETDB2 recombinant protein

Products by Pathway	Pathway Diagram
Lysine Degradation Pathway antibodies	Lysine Degradation Pathway Diagram
Lysine Degradation Pathway antibodies	Lysine Degradation Pathway Diagram

Diseases associated with SETDB2 recombinant protein

Disease Name	Pubmed Publications
Immune System Diseases Antibodies	>4 publications with SETDB2 and Immune System Diseases
Congenital Abnormalities Antibodies	>1 publications with SETDB2 and Congenital Abnormalities

Organs/Tissues associated with SETDB2 recombinant protein

Organ/Tissue Name	Pubmed Publications
Testis Antibodies	>1 publications with SETDB2 and Testis