• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

PEAK1 Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS515334

Recombinant human PEAK1 (1127-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.Scientific Background: PEAK1 or Pseudopodium-enriched atypical kinase 1 is a protein tyrosine kinase which belongs to NKF3 kinase family and… More details

$350 / 0.02 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PEAK1, Recombinant Protein
Full Product Name
PEAK1, Unactive recombinant protein
Product Gene Name
PEAK1 recombinant protein
Product Synonym Gene Name
SGK269
Product Synonym Names
SGK269
Host
Sf9 insect cells
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA. 0.25mM DTT, 0.1 mM PMSF, 25% glycerol.
Purity/Purification
>90%
Concentration
0.2 ug/ul (lot specific)
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Product shipped on dry ice.
Molecular Weight
~100 kDa
Chromosome Location
Chromosome: 15; NC_000015.9 (77400498..77712446, complement). Location: 15q24.3
OMIM
614248
3D Structure
ModBase 3D Structure for Q9H792
Accession Number
NM 024776
Type
Recombinant Fusion Protein
Species
Human
Tag Information
GST tag
Expression System
Sf9 insect cells using baculovirus
Source Note
Recombinant human PEAK1 (1127-end) was expressed by baculovirus in Sf9 insect cells
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.

Product Categories/Family for PEAK1 recombinant protein

Unactive Kinase; Signaling Proteins - Unactive Kinases
Applications Tested/Suitable for PEAK1 recombinant protein
Kinase Assay, Western Blot (WB)

SDS-PAGE of PEAK1 recombinant protein

The purity of PEAK1 was determined to be >95% by densitometry. Approx, MW: 100kDa
PEAK1 Recombinant Protein – Cat# MBS515334

Product References and Citations for PEAK1 recombinant protein

1. Wang, Y. et.al: Pseudopodium-enriched atypical kinase 1 regulates the cytoskeleton and cancer progression. Proc. Nat. Acad. Sci. 107: 10920-10925, 2010.
2. Ohara, O. et.al: Characterization of size-fractionated cDNA libraries generated by the in vitro recombination-assisted method. DNA Res. 9: 47-57, 2002.

Other Notes

Small volumes of PEAK1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PEAK1 purchase

MBS515334 is a recombinant protein targeting PEAK1. Also known as PEAK1, Unactive recombinant protein. Host: Sf9 insect cells.

Additional Details

NCBI/Uniprot data below describe general gene information for PEAK1. It may not necessarily be applicable to this product.

NCBI GI #
336176090
NCBI GeneID
79834
NCBI Accession #
NP_079052.2
UniProt Primary Accession #
Q9H792
UniProt Secondary Accession #
Q6ZS78; Q8NAZ4; Q8NCM3; Q8TEG7
UniProt Related Accession #
Q9H792
GenBank Nucleotide #
NM_024776
NCBI Official Full Name
Homo sapiens NKF3 kinase family member (PEAK1), mRNA
NCBI Official Symbol
PEAK1
NCBI Official Synonym Symbols
SGK269
UniProt Protein Name
Pseudopodium-enriched atypical kinase 1
UniProt Synonym Protein Names
Sugen kinase 269; Tyrosine-protein kinase SgK269
Protein Family
Pseudopodium-enriched atypical kinase
UniProt Gene Name
PEAK1
UniProt Synonym Gene Names
KIAA2002
UniProt Entry Name
PEAK1_HUMAN
NCBI Protein Information
pseudopodium-enriched atypical kinase 1; sugen kinase 269; tyrosine-protein kinase SgK269; pseudopodium enriched atypical kinase

NCBI Summary for PEAK1

This gene encodes a non-receptor tyrosine kinase that is a member of the new kinase family three (NFK3) family. In migrating cells, the encoded protein is associated with the actin cytoskeleton and focal adhesions and promotes developing focal adhesion elongation. This protein may play a role in the regulation of cell migration, proliferation and cancer metastasis. [provided by RefSeq, Mar 2014]
Show more

UniProt Comments for PEAK1

Function: Tyrosine kinase that may play a role in cell spreading and migration on fibronectin. May directly or indirectly affect phosphorylation levels of cytoskeleton-associated proteins MAPK1/ERK and PXN. Ref.7

Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. Ref.7

Subunit structure: Interacts with BCAR1 and CRK. Ref.7

Subcellular location: Cytoplasm › cytoskeleton. Cell junction › focal adhesion. Note: Colocalizes with F-actin in serum-rich medium. Actin colocalization is reduced during serum starvation. Ref.7

Post-translational modification: Phosphorylated on tyrosine in a CSK-dependent manner in response to adhesion to fibronectin and to EGF stimulation. Autophosphorylated in vitro. Ref.7

Sequence similarities: Belongs to the protein kinase superfamily.Contains 1 protein kinase domain.

Sequence caution: The sequence BAB15006.1 differs from that shown. Reason: Erroneous initiation. The sequence BAC87076.1 differs from that shown. Reason: Erroneous initiation.
Show more

Research Articles on PEAK1

1. results define a novel signaling pathway in basal breast cancer involving Lyn and SgK269 that offers clinical opportunities for therapeutic intervention

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PEAK1 recombinant protein

 Reference Product  PubMed Publications
 GRB2 recombinant protein  >2 publications with PEAK1 and GRB2
 CRK recombinant protein  >1 publications with PEAK1 and CRK

Diseases associated with PEAK1 recombinant protein

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >5 publications with PEAK1 and Neoplasms
 Scleroderma, Diffuse Antibodies  >1 publications with PEAK1 and Scleroderma, Diffuse
 Breast Neoplasms Antibodies  >1 publications with PEAK1 and Breast Neoplasms
 Liver Neoplasms Antibodies  >1 publications with PEAK1 and Liver Neoplasms
 Lymphoma Antibodies  >1 publications with PEAK1 and Lymphoma

Organs/Tissues associated with PEAK1 recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >3 publications with PEAK1 and Blood
 Liver Antibodies  >1 publications with PEAK1 and Liver
 Muscle Antibodies  >1 publications with PEAK1 and Muscle
 Brain Antibodies  >1 publications with PEAK1 and Brain
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000