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PAD4 Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS515750

Recombinant full-length human PAD4 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The gene accession number is BC025718.Scientific Background: PAD4 is a member of the peptidyl arginine deiminase family of enzymes, which catalyze… More details

$435 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PAD4, Active Protein
Full Product Name
PAD4, Active
Product Gene Name
Product Synonym Names
PADI4; PAD; PDI4; PDI5
Host
Sf9 Insect Cells
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Purity/Purification
>95%
Concentration
0.1ug/ul (lot specific)
Preparation and Storage
Store product at –70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.

Specific Activity of PAD4 active protein

The specific activity of PAD4 was determined to be 4.1 nmol/min/mg as per activity assay protocol.
PAD4 Active Protein – Cat# MBS515750

SDS-Page of PAD4 active protein

The purity of PAD4 was determined to be >95% by densitometry. Approx. MW 96 kDa.
PAD4 Active Protein, SDS-Page – Cat# MBS515750

Product References and Citations for PAD4 active protein

1. Nakashima, K. et.al: Molecular characterization of peptidylarginine deiminase in HL-60 cells induced by retinoic acid and 1-alpha,25-dihydroxyvitamin D(3). J. Biol. Chem. 274: 27786-27792, 1999.
2. Li, P.et.al: PAD4 is essential for antibacterial innate immunity mediated by neutrophil extracellular traps. J. Exp. Med. 207: 1853-1862, 2010.
3. Wang, Y. et.al: Human PAD4 regulates histone arginine methylation levels via demethylimination. Science 306: 279-283, 2004.

Other Notes

Small volumes of PAD4 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PAD4 purchase

MBS515750 is an active protein targeting PAD4. Also known as PAD4, Active. Host: Sf9 Insect Cells.

Additional Details

NCBI/Uniprot data below describe general gene information for PAD4. It may not necessarily be applicable to this product.

NCBI Accession #
Protein Family

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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