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IgG Fraction (RBC) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS524071

MBS524071 is rabbit IgG directed against rat red blood cells. This IgG fraction was isolated from a high titer antiserum by salt fractionation and DEAE ion exchange chromatography. It was titered against its immunogens, sterile filtered, and analyzed for… More details

$435 / 20 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
RBC, IgG Fraction, Polyclonal Antibody
Full Product Name
Anti-Rat RBC, IgG Fraction, (Polyclonal) (rabbit IgG)
Product Gene Name
anti-RBC antibody
Product Synonym Names
RBC, IgG Fraction, (Polyclonal) (rabbit IgG); Rabbit anti-Rat Red Blood Cell, IgG Fraction
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Rat
Concentration
Antibody Concentration: 10.2 mg IgG/ml (lot specific)
Preparation and Storage
Ships on dry ice. Divide into useful aliquots and store at -20 degree C. Remove samples under sterile conditions or add a preservative.
Presentation
20 mg of IgG fraction in sterile phosphate buffered saline.
Preservatives
None (0.2um filtered).
Applications Tested/Suitable for anti-RBC antibody
Cytotoxic Assay (CA) and Hemagglutination Assays (HA)
Application Notes for anti-RBC antibody
This reagent is suitable for use in cytotoxic assays. The highest dilution of the product that yields agglutination.

Test Type:
Hemagglutination:

Test Cell Suspension: 100 ul of washed fresh rat red blood cells in saline
Product Tested: Diluted antibody (lot # 0179A-G) in 100 ul of heat inactivated normal rabbit serum diluted 1:100 in saline.
Incubation: 37 degree C for 20 mins, in U-bottom microtiter plates
20 degree C for 2 to 4 hrs. for cell settling pattern
Titer: The highest dilution of the product that yields agglutination.
Results: Titer >1:400

Other Notes

Small volumes of anti-RBC antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RBC purchase

MBS524071 is an antibody targeting RBC. Also known as Anti-Rat RBC, IgG Fraction, (Polyclonal) (rabbit IgG). Reactive in Rat. Host: Rabbit.
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