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Parathyroid Hormone (PTH) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9600171

Description: PTH PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Belongs to the parathyroid hormone family.Function: PTH elevates calcium level by dissolving the salts in bone and preventing their renal… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Parathyroid Hormone (PTH), Polyclonal Antibody
Full Product Name
Parathyroid Hormone Antibody
Product Gene Name
anti-PTH antibody
Product Synonym Names
hPTH; Parathormone; Parathyrin; Parathyroid hormone 1; Parathyroid hormone; Prepro PTH; Preproparathyroid hormone; PTH; PTH1; PTH1 receptor; PTH1R; PTHR; PTHR1; PTHY_HUMAN
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Concentration
1mg/ml (lot specific)
Specificity
Parathyroid Hormone antibody detects endogenous levels of total Parathyroid Hormone
Preparation and Storage
Store at -20°C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
Parathyroid Hormone peptide (MBS9614723) is used for blocking the activity of Parathyroid Hormone antibody (MBS9600171)
Molecular Weight
16kDa
3D Structure
ModBase 3D Structure for P01270
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human Parathyroid Hormone, corresponding to a region within the internal amino acids.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-PTH antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-PTH antibody
WB: 1:500-1:3000,
IF/ICC: 1:100-1:500,
ELISA(peptide): 1:20000-1:40000

Western Blot (WB) of anti-PTH antibody

Western blot analysis on A549 cell lysate using Parathyroid Hormone Antibody. The lane on the left is treated with the antigen-specific peptide.
Rabbit Anti-PTH Polyclonal Antibody – Cat# MBS9600171

Immunofluorescene (IF) of anti-PTH antibody

MBS9600171 staining A549 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
Rabbit Anti-PTH Polyclonal Antibody, Immunofluorescene (IF) – Cat# MBS9600171

Immunofluorescence (IF) of anti-PTH antibody

MBS9600171 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9600171 1:200) and mouse anti-beta tubulin Ab(MBS9610328 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).
Rabbit Anti-PTH Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS9600171

Other Notes

Small volumes of anti-PTH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PTH purchase

MBS9600171 is an antibody targeting PTH. Also known as Parathyroid Hormone Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PTH. It may not necessarily be applicable to this product.

NCBI GI #
4506267
NCBI GeneID
5741
NCBI Accession #
NP_000306.1
UniProt Primary Accession #
P01270
UniProt Secondary Accession #
Q4VB48; Q9UD38
UniProt Related Accession #
P01270
GenBank Nucleotide #
NM_000315.3
NCBI Official Full Name
parathyroid hormone isoform 1 preproprotein
NCBI Official Synonym Full Names
parathyroid hormone
NCBI Official Symbol
PTH
NCBI Official Synonym Symbols
PTH1
UniProt Protein Name
Parathyroid hormone
UniProt Synonym Protein Names
Parathormone; Parathyrin
Protein Family
Parathyroid hormone
UniProt Gene Name
PTH
UniProt Synonym Gene Names
PTH
NCBI Protein Information
parathyroid hormone

NCBI Summary for PTH

This gene encodes a member of the parathyroid family of proteins. The encoded preproprotein is proteolytically processed to generate a protein that binds to the parathyroid hormone/parathyroid hormone-related peptide receptor and regulates blood calcium and phosphate levels. Excess production of the encoded protein, known as hyperparathyroidism, can result in hypercalcemia and kidney stones. On the other hand, defective processing of the encoded protein may lead to hypoparathyroidism, which can result in hypocalcemia and numbness. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]
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UniProt Comments for PTH

PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.

Research Articles on PTH

1. Dkk-1 and PTH levels in psoriatic arthritis are lower than healthy female controls, in contrast with rheumatoid arthritis, in which they are increased

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PTH antibody

 Reference Product  PubMed Publications
 PTH1R antibody  >188 publications with PTH and PTH1R
 PTH2R antibody  >16 publications with PTH and PTH2R

Pathways associated with anti-PTH antibody

 Products by Pathway  Pathway Diagram
 Class B/2 (Secretin Family Receptors) Pathway antibodies  Class B/2 (Secretin Family Receptors) Pathway Diagram
 Endochondral Ossification Pathway antibodies  Endochondral Ossification Pathway Diagram
 G Alpha (s) Signalling Events Pathway antibodies  G Alpha (s) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Osteoblast Signaling Pathway antibodies  Osteoblast Signaling Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By GPCR Pathway antibodies  Signaling By GPCR Pathway Diagram
 Vitamin D Synthesis Pathway antibodies  Vitamin D Synthesis Pathway Diagram

Diseases associated with anti-PTH antibody

 Disease Name  Pubmed Publications
 Hyperparathyroidism Antibodies  >4851 publications with PTH and Hyperparathyroidism
 Bone Diseases Antibodies  >4134 publications with PTH and Bone Diseases
 Kidney Diseases Antibodies  >3096 publications with PTH and Kidney Diseases
 Hyperparathyroidism, Secondary Antibodies  >2664 publications with PTH and Hyperparathyroidism, Secondary
 Bone Diseases, Metabolic Antibodies  >2493 publications with PTH and Bone Diseases, Metabolic
 Hypercalcemia Antibodies  >2294 publications with PTH and Hypercalcemia
 Bone Resorption Antibodies  >2146 publications with PTH and Bone Resorption
 Renal Insufficiency Antibodies  >1972 publications with PTH and Renal Insufficiency
 Osteoporosis Antibodies  >1891 publications with PTH and Osteoporosis
 Kidney Failure, Chronic Antibodies  >1708 publications with PTH and Kidney Failure, Chronic

Organs/Tissues associated with anti-PTH antibody

 Organ/Tissue Name  Pubmed Publications
 Parathyroid Antibodies  >14515 publications with PTH and Parathyroid
 Connective Tissue Antibodies  >2270 publications with PTH and Connective Tissue
 Embryonic Tissue Antibodies  >229 publications with PTH and Embryonic Tissue
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