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PERK Antibody – Chicken Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS5400780

Overview: Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of… More details

$610 / 0.1 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
PERK, Polyclonal Antibody
Full Product Name
PERK Antibody FITC
Product Gene Name
Product Synonym Names
FITC-Conjugated Eukaryotic translation initiation factor 2-alpha kinase 3 Antibody; DKFZp781H1925 antibody; E2AK3_HUMAN antibody; EC 2.7.11.1 antibody; Eif2ak3 antibody; Eukaryotic translation initiation factor 2 alpha kinase 3 antibody; Heme regulated EIF2 alpha kinase antibody; HRI antibody; HsPEK antibody; Pancreatic eIF2 alpha kinase antibody; PEK antibody; PRKR like endoplasmic reticulum kinase antibody; WRS antibody
Clonality
Polyclonal
Isotype
IgY
Host
Chicken
Species Reactivity
Human, Mouse, Rat
Concentration
0.68-0.70 ug/ul in antibody stabilization buffer (lot specific)
Preparation and Storage
Store at -20 degree C for long term storage.
Molecular Weight
125,216 Da
3D Structure
ModBase 3D Structure for Q9NZJ5
Immunogen
Synthetic peptide taken within amino acid region 1000-1050 on human Eukaryotic translation initiation factor 2-alpha kinase 3 protein.
Conjugation
FITC
Molecular Function
Kinase; Serine/threonine-protein kinase; Transferase
Subcellular Location
Endoplasmic reticulum membrane; Single-pass type I membrane protein
Applications Tested/Suitable for anti-PERK antibody
Dot Blot (DB), ELISA (EIA), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-PERK antibody
DB: 1:4,000
ELISA: 1:4,000
IHC: 1:50-1:100
IP: 1:150
WB: 1:500

Other Notes

Small volumes of anti-PERK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PERK purchase

MBS5400780 is an antibody targeting PERK. Also known as PERK Antibody FITC. Reactive in Human, Mouse, Rat. Host: Chicken.

Additional Details

NCBI/Uniprot data below describe general gene information for PERK. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
eukaryotic translation initiation factor 2-alpha kinase 3 isoform 1
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 2 alpha kinase 3
NCBI Official Symbol
EIF2AK3
NCBI Official Synonym Symbols
PEK; WRS; PERK
UniProt Protein Name
Eukaryotic translation initiation factor 2-alpha kinase 3
UniProt Synonym Protein Names
PRKR-like endoplasmic reticulum kinase; Pancreatic eIF2-alpha kinase; HsPEK
UniProt Gene Name
EIF2AK3
UniProt Synonym Gene Names
PEK; PERK; HsPEK
NCBI Protein Information
eukaryotic translation initiation factor 2-alpha kinase 3

NCBI Summary for PERK

The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Sep 2015]
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UniProt Comments for PERK

Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2S1) on 'Ser-52' during the unfolded protein response (UPR) and in response to low amino acid availability. Converts phosphorylated eIF-2-alpha/EIF2S1 either in a global protein synthesis inhibitor, leading to a reduced overall utilization of amino acids, or to a translation initiation activator of specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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