Lysozyme

Lysozyme is a single chain polypeptide of 129 amino acids cross-linked with four disulfide bridges. It hydrolyzes BETA(1?4) linkages between N-acetylmuraminic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. The enzyme is often used for lysing bacterial cells by hydrolyzing the peptidoglycan present in the cell walls. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gramnegative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed more easily in the presence of EDTA that chelates metal ions in the outer bacterial membrane.

This lysozyme preparation is purified, crystallized three times, dialyzed, and supplied as a lyophilized powder. Protein content by UV absorbance is ?90% with the remainder (~10%) being buffer salts such as sodium acetate and sodium chloride.

This highly purified enzyme preparation has been used in mass spectrometry as a protein mass calibration standard and in structural studies of proteins. It is suitable for use as a lysing agent in the purification of plasmid DNA using a boiling lysing technique.

Small volumes of Lysozyme biochemical vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS545614 is a biochemical targeting Lysozyme.