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Stem Cell Factor (SCF) Mouse Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS553178

SCF is a stromal cell- derived cytokine synthesized by fibroblasts and other cell types. SCF promotes proliferation and early differentiation of cells at the level of multipotential stem cells. It has been suggested that SCF is essential for optimal… More details

$335 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Stem Cell Factor (SCF), Recombinant Protein
Full Product Name
Mouse Stem Cell Factor Recombinant
Product Gene Name
SCF recombinant protein
Product Synonym Names
Mouse Stem cell Factor; kit ligand; kitlg; mgf; stem cell factor; scf
Sequence
MKKTQTWIIT CIYLQLLLFN PLVKTKEICG NPVTDNVKDI TKLVANLPND YMITLNYVAG MDVLPSHCWL RDMVIQLSLS LTTLLDKFSN ISEGLSNYSI IDKLGKIVDD LVLCMEENAP KNIKESPKRP ETRSFTPEEF FSIFNRSIDA FKDFMVASDT SDCVLSSTLG PEKDSRVSVT KPFMLPPVAA SSLRNDSSSS NRKAAKAPED SGLQWTAMAL PALISLVIGF AFGALYWKKK QSSLTRAVEN IQINEEDNEI SMLQQKEREF QEV
Host
Insect Cells (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders)
Form/Format
Recombinant Stem cell factor was lyophilized from a 0.2 um filtered solution in 20mM Tris, 5% trehalose, pH 7.5.
Purity/Purification
>95%, as determined by SDS-PAGE and HPLC
Preparation and Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.
Molecular Weight
30,645 Da
3D Structure
ModBase 3D Structure for P20826
Domain
Four helix core
Host Note
Optimized DNA sequence encoding Mouse SCF mature chain was expressed in Insect cells.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Molecular Weight Note
Native Mouse Stem Cell Factor is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 18 kDa.

Recombinant SCF is a disulfide-linked monomer protein consisting of 165 amino acid residue subunits, and due to glycosylation migrates as an approximately 23 kDa protein under non-reducing and reducing conditions in SDS-PAGE.

Product Categories/Family for SCF recombinant protein

Cytokines

Other Notes

Small volumes of SCF recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SCF purchase

MBS553178 is a recombinant protein targeting SCF. Also known as Mouse Stem Cell Factor Recombinant. Host: Insect Cells (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders).

Additional Details

NCBI/Uniprot data below describe general gene information for SCF. It may not necessarily be applicable to this product.

NCBI GI #
7305267
NCBI GeneID
17311
NCBI Accession #
NP_038626.1
UniProt Primary Accession #
P20826
UniProt Secondary Accession #
P97332; Q3TNJ7; Q62524; Q64222; Q921N5
UniProt Related Accession #
P20826; Q61854; Q64384; Q78ED8; Q8C9K1
GenBank Nucleotide #
NM_013598.2
NCBI Official Full Name
kit ligand
UniProt Protein Name
Kit ligand
UniProt Synonym Protein Names
Hematopoietic growth factor KL; Mast cell growth factor; MGF; Steel factor; Stem cell factor; SCF; c-Kit ligand
Protein Family
Kit ligand
UniProt Gene Name
Kitlg
UniProt Synonym Gene Names
Kitl; Mgf; Sl; Slf; MGF; SCF; sKITLG
UniProt Entry Name
SCF_MOUSE

UniProt Comments for SCF

SCF: Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5- trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins. Homodimer, non-covalently linked (Probable). Heterotetramer with KIT, binding two KIT molecules; thereby mediates KIT dimerization and subsequent activation by autophosphorylation. Belongs to the SCF family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: extracellular space; cytoskeleton; membrane; cytoplasm; integral to membrane; extracellular region; plasma membrane

Molecular Function: protein binding; growth factor activity; cytokine activity; stem cell factor receptor binding

Biological Process: embryonic hemopoiesis; positive regulation of leukocyte migration; germ cell programmed cell death; germ cell development; positive regulation of MAP kinase activity; positive regulation of peptidyl-tyrosine phosphorylation; ovarian follicle development; positive regulation of melanocyte differentiation; negative regulation of mast cell apoptosis; positive regulation of cell proliferation; positive regulation of Ras protein signal transduction; positive regulation of myeloid leukocyte differentiation; cell adhesion; neural crest cell migration; positive regulation of DNA replication; negative regulation of apoptosis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with SCF recombinant protein

 Products by Pathway  Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Kit Receptor Signaling Pathway antibodies  Kit Receptor Signaling Pathway Diagram
 Melanogenesis Pathway antibodies  Melanogenesis Pathway Diagram
 Melanogenesis Pathway antibodies  Melanogenesis Pathway Diagram
 Pathways In Cancer antibodies  Pathways In Cancer Diagram

Diseases associated with SCF recombinant protein

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >8 publications with SCF and Neoplasms
 Female Urogenital Diseases Antibodies  >5 publications with SCF and Female Urogenital Diseases
 Skin Diseases Antibodies  >4 publications with SCF and Skin Diseases
 Infertility Antibodies  >4 publications with SCF and Infertility
 Gonadal Disorders Antibodies  >4 publications with SCF and Gonadal Disorders
 Inflammation Antibodies  >4 publications with SCF and Inflammation
 Drug Toxicity Antibodies  >3 publications with SCF and Drug Toxicity
 Melanoma Antibodies  >3 publications with SCF and Melanoma
 Leukemia Antibodies  >3 publications with SCF and Leukemia
 Infertility, Male Antibodies  >2 publications with SCF and Infertility, Male

Organs/Tissues associated with SCF recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >23 publications with SCF and Bone
 Ovary Antibodies  >18 publications with SCF and Ovary
 Skin Antibodies  >17 publications with SCF and Skin
 Bone Marrow Antibodies  >12 publications with SCF and Bone Marrow
 Testis Antibodies  >10 publications with SCF and Testis
 Embryonic Tissue Antibodies  >7 publications with SCF and Embryonic Tissue
 Liver Antibodies  >6 publications with SCF and Liver
 Heart Antibodies  >4 publications with SCF and Heart
 Spleen Antibodies  >3 publications with SCF and Spleen
 Eye Antibodies  >2 publications with SCF and Eye
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