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SPARC Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS553651

MBS553651 is a recombinant protein targeting SPARC. Host: Human (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders). More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SPARC, Recombinant Protein
Full Product Name
SPARC
Product Gene Name
SPARC recombinant protein
Sequence
APQQEALPDE TEVVEETVAE VTEVSVGANP VQVEVGEFDD GAEETEEEVV AENPCQNHHC KHGKVCELDE NNTPMCVCQD PTSCPAPIGE FEKVCSNDNK TFDSSCHFFA TKCTLEGTKK GHKLHLDYIG PCKYIPPCLD SELTEFPLRM RDWLKNVLVT LYERDEDNNL LTEKQKLRVK KIHENEKRLE AGDHPVELLA RDFEKNYNMY IFPVHWQFGQ LDQHPIDGYL SHTELAPLRA PLIPMEHCTT RFFETCDLDN DKYIALDEWA GCFGIKQKDI DKDLVI
Host
Human (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders)
Form/Format
Recombinant human SPARC is lyophilized from 0.2um filtered 10mM Sodium Phosphate , pH 7.6
Purity/Purification
>95%, as determined by SDS-PAGE
Preparation and Storage
Store lyophilized product at room tempeture for 3 weeks or at -20°C for 12 months.
Store reconstituted product at 2-8°C for 3 weeks or at -20°C for 12 months.
*For long term storage we recommend the addition of a carrier protein at 0.1%.
*Avoid repeated freeze/thaw cycles.
3D Structure
ModBase 3D Structure for P09486
Source Note
DNA sequence encoding the mature chain Human SPARC was expressed in CHO cells.
Molecular Weight Note
Recombinant Human SPARC is a protein consisting of 286 amino acid residues, and migrates as an approximateley 43 kDa protein on SDS-PAGE.
Activity
The bilogical activity was determined by the ability to induce alkaline phosphatase activity in Mouse 3T3 with linear range of 0.3-0.6 ug/mL
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended 20 sec. , 5K RPM. Follow by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. If further dilutions are required it is recommended to dilute product in the formulation buffer as given in the presentation.
Applications Tested/Suitable for SPARC recombinant protein
SDS-PAGE
Application Notes for SPARC recombinant protein
Recombinant SPARC migrates as an approximately 31 kDa protein under reducing conditions in SDS-PAGE.

Other Notes

Small volumes of SPARC recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SPARC. It may not necessarily be applicable to this product.

NCBI GI #
4507171
NCBI GeneID
6678
NCBI Accession #
NP_003109.1
UniProt Primary Accession #
P09486
UniProt Secondary Accession #
Q6IBK4; D3DQH9
UniProt Related Accession #
P09486
GenBank Nucleotide #
NM_003118.4
NCBI Official Full Name
SPARC isoform 1
NCBI Official Synonym Full Names
secreted protein acidic and cysteine rich
NCBI Official Symbol
SPARC
NCBI Official Synonym Symbols
ON; OI17; BM-40
UniProt Protein Name
SPARC
UniProt Synonym Protein Names
Basement-membrane protein 40; BM-40; Osteonectin; ON; Secreted protein acidic and rich in cysteine
Protein Family
SPARC
UniProt Gene Name
SPARC
UniProt Synonym Gene Names
ON; BM-40; ON
NCBI Protein Information
SPARC

NCBI Summary for SPARC

This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2015]
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UniProt Comments for SPARC

Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca2+ with a low affinity and an EF-hand loop that binds a Ca2+ ion with a high affinity.
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Research Articles on SPARC

1. Hepatic SPARC expression is associated with liver injury and fibrogenic processes in non-alcoholic fatty liver disease.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with SPARC recombinant protein

 Reference Product  PubMed Publications
 TGFB1 recombinant protein  >71 publications with SPARC and TGFB1
 COL1A2 recombinant protein  >33 publications with SPARC and COL1A2
 COL1A1 recombinant protein  >30 publications with SPARC and COL1A1
 EGF recombinant protein  >26 publications with SPARC and EGF
 SERPINE1 recombinant protein  >18 publications with SPARC and SERPINE1
 FN1 recombinant protein  >15 publications with SPARC and FN1
 TIMP1 recombinant protein  >7 publications with SPARC and TIMP1
 PLG recombinant protein  >2 publications with SPARC and PLG
 ALB recombinant protein  >1 publications with SPARC and ALB

Pathways associated with SPARC recombinant protein

 Products by Pathway  Pathway Diagram
 Binding And Uptake Of Ligands By Scavenger Receptors Pathway antibodies  Binding And Uptake Of Ligands By Scavenger Receptors Pathway Diagram
 ECM Proteoglycans Pathway antibodies  ECM Proteoglycans Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Platelet Activation, Signaling And Aggregation Pathway antibodies  Platelet Activation, Signaling And Aggregation Pathway Diagram
 Platelet Degranulation Pathway antibodies  Platelet Degranulation Pathway Diagram
 Response To Elevated Platelet Cytosolic Ca2+ Pathway antibodies  Response To Elevated Platelet Cytosolic Ca2+ Pathway Diagram
 Scavenging By Class H Receptors Pathway antibodies  Scavenging By Class H Receptors Pathway Diagram
 Senescence And Autophagy Pathway antibodies  Senescence And Autophagy Pathway Diagram
 TGF-beta Receptor Signaling Pathway antibodies  TGF-beta Receptor Signaling Pathway Diagram

Diseases associated with SPARC recombinant protein

 Disease Name  Pubmed Publications
 Adenocarcinoma Antibodies  >111 publications with SPARC and Adenocarcinoma
 Cardiovascular Diseases Antibodies  >109 publications with SPARC and Cardiovascular Diseases
 Disease Models, Animal Antibodies  >103 publications with SPARC and Disease Models, Animal
 Inflammation Antibodies  >102 publications with SPARC and Inflammation
 Fibrosis Antibodies  >94 publications with SPARC and Fibrosis
 Brain Diseases Antibodies  >76 publications with SPARC and Brain Diseases
 Breast Neoplasms Antibodies  >74 publications with SPARC and Breast Neoplasms
 Necrosis Antibodies  >61 publications with SPARC and Necrosis
 Neoplasms, Experimental Antibodies  >53 publications with SPARC and Neoplasms, Experimental
 Lung Neoplasms Antibodies  >47 publications with SPARC and Lung Neoplasms

Organs/Tissues associated with SPARC recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >1082 publications with SPARC and Bone
 Connective Tissue Antibodies  >445 publications with SPARC and Connective Tissue
 Blood Antibodies  >332 publications with SPARC and Blood
 Vascular Antibodies  >300 publications with SPARC and Vascular
 Bone Marrow Antibodies  >202 publications with SPARC and Bone Marrow
 Muscle Antibodies  >181 publications with SPARC and Muscle
 Embryonic Tissue Antibodies  >173 publications with SPARC and Embryonic Tissue
 Mouth Antibodies  >159 publications with SPARC and Mouth
 Brain Antibodies  >151 publications with SPARC and Brain
 Eye Antibodies  >121 publications with SPARC and Eye
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