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TNFSF14 Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS553712

MBS553712 is an active protein targeting TNFSF14. Host: E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders). More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
TNFSF14, Active Protein
Full Product Name
TNFSF14
Product Gene Name
TNFSF14 active protein
Sequence
MRLHQRLGDI VAHLPDGGKG SWEKLIQDQR SHQANPAAHL TGANASLIGI GGPLLWETRL GLAFLRGLTY HDGALVTMEP GYYYVYSKVQ LSGVGCPQGL ANGLPITHGL YKRTSRYPKE LELLVSRRSP CGRANSSRVW WDSSFLGGVV HLEAGEEVVV RVPGNRLVRP RDGTRSYFGA
Host
E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders)
Form/Format
Lyophilized; PBS, pH 7.2
Purity/Purification
>95%, as determined by SDS-PAGE and HPLC.
Preparation and Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C. Upon reconstitution, this product can be stored in working aliquots at 2 degree C - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Molecular Weight
22,395 Da
3D Structure
ModBase 3D Structure for O43557
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Species
Mouse
Source Note
Optimized DNA sequence encoding Mouse TNFSF14 mature chain was expressed in ECOLI. Recombinant Mouse Tumor necrosis factor ligand superfamily member 14 encodes amino acid residues 1-239.
Biological Activity
Determined by its cytotoxic effect on HT-29 cells (human colon adenocarcinoma cells) in the presence of human IFN-.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug(1EU/ug).
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Applications Tested/Suitable for TNFSF14 active protein
SDS-PAGE
Application Notes for TNFSF14 active protein
Recombinant TNFSF14 migrates as an approximately 20 kDa protein under reducing conditions in SDS-PAGE.

Other Notes

Small volumes of TNFSF14 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TNFSF14. It may not necessarily be applicable to this product.

NCBI GI #
291045244
NCBI GeneID
8740
NCBI Accession #
NP_742011.2
UniProt Primary Accession #
O43557
UniProt Secondary Accession #
O75476; Q6FHA1; Q8WVF8; Q96LD2; A8K7M2; C9J5H4
UniProt Related Accession #
O43557
GenBank Nucleotide #
NM_172014.3
NCBI Official Full Name
tumor necrosis factor ligand superfamily member 14 isoform 2
NCBI Official Synonym Full Names
TNF superfamily member 14
NCBI Official Symbol
TNFSF14
NCBI Official Synonym Symbols
LTg; CD258; HVEML; LIGHT
UniProt Protein Name
Tumor necrosis factor ligand superfamily member 14
UniProt Synonym Protein Names
Herpes virus entry mediator ligand; HVEM-L; Herpesvirus entry mediator ligand
Protein Family
Tumor necrosis factor ligand superfamily
UniProt Gene Name
TNFSF14
UniProt Synonym Gene Names
HVEML; LIGHT; HVEM-L; Herpesvirus entry mediator ligand
NCBI Protein Information
tumor necrosis factor ligand superfamily member 14

NCBI Summary for TNFSF14

The protein encoded by this gene is a member of the tumor necrosis factor (TNF) ligand family. This protein is a ligand for TNFRSF14, which is a member of the tumor necrosis factor receptor superfamily, and which is also known as a herpesvirus entry mediator (HVEM). This protein may function as a costimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells. This protein is also reported to prevent tumor necrosis factor alpha mediated apoptosis in primary hepatocyte. Two alternatively spliced transcript variant encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
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UniProt Comments for TNFSF14

Cytokine that binds to TNFRSF3/LTBR. Binding to the decoy receptor TNFRSF6B modulates its effects. Activates NFKB, stimulates the proliferation of T-cells, and inhibits growth of the adenocarcinoma HT-29. Acts as a receptor for Herpes simplex virus.

Research Articles on TNFSF14

1. the outcomes of this study provide compelling evidence that TNFSF14 is necessary to limit relevant steps in the pathogenesis of the metabolic syndrome and support the development of agonists of TNFSF14 signaling as attractive therapeutics for treating obesity and type 2 diabetes

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with TNFSF14 active protein

 Reference Product  PubMed Publications
 TNFRSF14 active protein  >67 publications with TNFSF14 and TNFRSF14
 LTBR active protein  >40 publications with TNFSF14 and LTBR
 TNFRSF6B active protein  >16 publications with TNFSF14 and TNFRSF6B
 BTLA active protein  >13 publications with TNFSF14 and BTLA
 TRAF2 active protein  >6 publications with TNFSF14 and TRAF2
 TRAF3 active protein  >5 publications with TNFSF14 and TRAF3
 DIABLO active protein  >2 publications with TNFSF14 and DIABLO
 BIRC2 active protein  >1 publications with TNFSF14 and BIRC2

Pathways associated with TNFSF14 active protein

 Products by Pathway  Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Herpes Simplex Infection Pathway antibodies  Herpes Simplex Infection Pathway Diagram
 Herpes Simplex Infection Pathway antibodies  Herpes Simplex Infection Pathway Diagram
 NF-kappa B Signaling Pathway antibodies  NF-kappa B Signaling Pathway Diagram

Diseases associated with TNFSF14 active protein

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >235 publications with TNFSF14 and Necrosis
 Inflammation Antibodies  >62 publications with TNFSF14 and Inflammation
 Liver Diseases Antibodies  >15 publications with TNFSF14 and Liver Diseases
 Neoplasms, Experimental Antibodies  >11 publications with TNFSF14 and Neoplasms, Experimental
 Arthritis, Rheumatoid Antibodies  >10 publications with TNFSF14 and Arthritis, Rheumatoid
 Lung Diseases Antibodies  >8 publications with TNFSF14 and Lung Diseases
 Breast Neoplasms Antibodies  >5 publications with TNFSF14 and Breast Neoplasms
 Atherosclerosis Antibodies  >5 publications with TNFSF14 and Atherosclerosis
 Liver Neoplasms Antibodies  >5 publications with TNFSF14 and Liver Neoplasms
 Heart Diseases Antibodies  >4 publications with TNFSF14 and Heart Diseases

Organs/Tissues associated with TNFSF14 active protein

 Organ/Tissue Name  Pubmed Publications
 Spleen Antibodies  >22 publications with TNFSF14 and Spleen
 Liver Antibodies  >18 publications with TNFSF14 and Liver
 Brain Antibodies  >7 publications with TNFSF14 and Brain
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