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Anti-Guinea Pig IgA Antibody – Sheep Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS560633

Sheep were immunized with highly purified guinea pig IgA. The resulting antiserum was passed over an antigen containing immunoabsorbant from which the anti-guinea pig IgA was immunoaffinity purified. More details

$890 / 1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
anti-Guinea Pig IgA, Polyclonal Antibody
Full Product Name
Affinity Purified Sheep anti-Guinea Pig IgA
Product Synonym Names
Affinity Purified Sheep anti-Guinea Pig IgA
Clonality
Polyclonal
Host
Sheep
Species Reactivity
This antibody reacts with guinea pig IgA as determined by IEP and ELISA techniques.
Form/Format
Liquid
Purity/Purification
Unconjugated A.P.
Concentration
1.0mL @ 1.0mg/mL (lot specific)
Preparation and Storage
2-8°C
Preservative
0.09% sodium azide
Buffer
Phosphate buffered saline, pH 7.2
Procedure
Sheep were immunized with highly purified guinea pig IgA and the resulting antiserum was collected. Antibody was solid phase adsorbed and immunoaffinity purified off a guinea pig IgA containing immunosorbent. Antibody concentration was determined using an absorbance at 280 nm: 1.4 equals 1.0 mg of IgG.
Cross Reactivity
This antibody may cross react with IgA from other species.
Immunogen
Guinea Pig IgA
Quality Control Testing
Quality control tested by Indirect ELISA utilizing the following parameters.
Step Action Materials Used
1 Antigen - 500 ng/well. Incubated 60 minutes Guinea Pig IgA
2 Block plates. Incubated 30 minutes 1% BSA
3 Primary Antibody. Incubated 60 minutes Affinity Purified Sheep anti-Guinea Pig IgA
4 HRP Conjugated Antibody. Incubated 30 minutes HRP Conjugated Donkey anti-Sheep IgG h+l (DGHL-35P)
5 Develop with TMB. Incubated 10 minutes TMB Substrate
6 Stop reaction 0.3M H2SO4
7 Read plates at A450 Microplate Reader set at 450 nm
ELISA Raw Data
Well Number Concentration A450
1 500 ng/mL 3.708
2 250 ng/mL 3.469
3 125 ng/mL 2.623
4 62.5 ng/mL 1.758
5 31.3 ng/mL 1.132
6 15.7 ng/mL 0.743
7 7.9 ng/mL 0.416
8 4.0 ng/mL 0.238
9 0 ng/mL 0.116
Applications Tested/Suitable for anti-anti-Guinea Pig IgA antibody
It is suitable for blotting, ELISA and IHC applications
Application Notes for anti-anti-Guinea Pig IgA antibody
Optimal working dilutions should be determined experimentally by the investigator.

Other Notes

Small volumes of anti-anti-Guinea Pig IgA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for anti-Guinea Pig IgA purchase

MBS560633 is an antibody targeting anti-Guinea Pig IgA. Also known as Affinity Purified Sheep anti-Guinea Pig IgA. Reactive in This antibody reacts with guinea pig IgA as determined by IEP and ELISA techniques.. Host: Sheep.

Additional Details

NCBI/Uniprot data below describe general gene information for anti-Guinea Pig IgA. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Official Full Name
IgA

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-anti-Guinea Pig IgA antibody

 Reference Product  PubMed Publications
 FLNA antibody  >12 publications with anti-Guinea Pig IgA and FLNA
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