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LRPAP1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200307

Alpha-2-macroglobulin receptor-associated protein, also known as low density lipoprotein receptor-related protein-associated protein 1, RAP and LRPAP1, is a 39 kDa protein and a member of the alpha-2-MRAP family. LRPAP1 is a receptor antagonist that interacts… More details

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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
LRPAP1, Monoclonal Antibody
Full Product Name
LRPAP1 antibody
Product Gene Name
Product Synonym Names
alpha-2-macroglobulin receptor-associated protein; A2MRAP; A2RAP; HBP44; MGC138272; MRAP; RAP
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
AT8G8
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-A affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
41,466 Da
OMIM
M63959 mRNA
3D Structure
ModBase 3D Structure for P30533
Antigen Species
Human
Immunogen
Recombinant human LRPAP1 (35-357aa) purified from E. coli
Cross reactivity
mouse
Clone Note
Anti-human LRPAP1 mAb, clone AT8G8, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human LRPAP1 protein.
Applications Tested/Suitable for anti-LRPAP1 antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-LRPAP1 antibody
Recommended starting dilution is 1:500.

Western Blot (WB) of anti-LRPAP1 antibody

The lysate of Mouse kidney (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human LRPAP1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Mouse Anti-LRPAP1 Monoclonal Antibody – Cat# MBS200307

Immunofluorescence (IF) of anti-LRPAP1 antibody

ICC/IF analysis of LRPAP in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human LRPAP1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Mouse Anti-LRPAP1 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200307

Product References and Citations for anti-LRPAP1 antibody

Kounnas. M.Z., et al. (1992) J Biol Chem 267 (29): 21162-21166; Bu. G., et al. (1995) EMBO J 14 (10): 2269-2280

Other Notes

Small volumes of anti-LRPAP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LRPAP1 purchase

MBS200307 is an antibody targeting LRPAP1. Also known as LRPAP1 antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for LRPAP1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
alpha-2-macroglobulin receptor-associated protein
NCBI Official Synonym Full Names
LDL receptor related protein associated protein 1
NCBI Official Symbol
LRPAP1
NCBI Official Synonym Symbols
RAP; MRAP; A2RAP; HBP44; MYP23; A2MRAP; alpha-2-MRAP
UniProt Protein Name
Alpha-2-macroglobulin receptor-associated protein
UniProt Synonym Protein Names
Low density lipoprotein receptor-related protein-associated protein 1; RAP
UniProt Gene Name
LRPAP1
UniProt Synonym Gene Names
A2MRAP; Alpha-2-MRAP; RAP
NCBI Protein Information
alpha-2-macroglobulin receptor-associated protein

NCBI Summary for LRPAP1

This gene encodes a protein that interacts with the low density lipoprotein (LDL) receptor-related protein and facilitates its proper folding and localization by preventing the binding of ligands. Mutations in this gene have been identified in individuals with myopia 23. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]
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UniProt Comments for LRPAP1

Molecular chaperone for LDL receptor-related proteins that may regulate their ligand binding activity along the secretory pathway.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-LRPAP1 antibody

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