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Histone H3 (HIST1H3A) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS565223

The nucleosome is made up of four core histone proteins (H2A, H2B, H3 and H4) and is the primary building block of chromatin. The core histones form an octamer, which is made up of one H3-H4 tetramer and two H2A-H2B dimers. The N-terminal tail of core… More details

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Product Overview

Product Name
Histone H3 (Hist1h3a), Polyclonal Antibody
Full Product Name
Histone H3 (acetyl-Lys9) polyclonal antibody
Product Gene Name
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Mouse
Weakly cross-reacts with human, rat, bovine and dog Histone H3 (acetyl-Lys9).
Form/Format
Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Purity/Purification
Peptide Affinity Purified
Specificity
Recognizes mouse, human (weak), rat (weak), bovine (weak) and dog (weak) Histone H3 (acetyl-Lys9).
Preparation and Storage
Long term storage: -20 degree C
Avoid freeze/thaw cycles.
Molecular Weight
15,404 Da
3D Structure
ModBase 3D Structure for P68433
Immunogen
Synthetic acetyl-peptide corresponding to the N-terminus of mouse histone H3.
Applications Tested/Suitable for anti-Hist1h3a antibody
Western Blot (WB)
Application Notes for anti-Hist1h3a antibody
Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.

Western Blot (WB) of anti-Hist1h3a antibody

Western blot analysis of Histone H3 (Acetyl-Lys9) using 3T3 cell extracts treated with TSA. Lane 1: Molecular Weight Markers, Lane 2: 3T3, Lane 3: 3T3 + TSA.
Rabbit Anti-HIST1H3A Polyclonal Antibody – Cat# MBS565223

Other Notes

Small volumes of anti-Hist1h3a antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Hist1h3a purchase

MBS565223 is an antibody targeting Hist1h3a. Also known as Histone H3 (acetyl-Lys9) polyclonal antibody. Reactive in Mouse
Weakly cross-reacts with human, rat, bovine and dog Histone H3 (acetyl-Lys9).. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Hist1h3a. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Histone H3.1
NCBI Official Synonym Full Names
histone cluster 1, H3a
NCBI Official Symbol
Hist1h3a
UniProt Protein Name
Histone H3.1
UniProt Gene Name
Hist1h3a
UniProt Synonym Gene Names
H3a
UniProt Entry Name
H31_MOUSE
NCBI Protein Information
histone H3.1

NCBI Summary for Hist1h3a

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. [provided by RefSeq, Aug 2015]
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UniProt Comments for Hist1h3a

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Hist1h3a antibody

 Reference Product  PubMed Publications
 Hist4h4 antibody  >1 publications with Hist1h3a and Hist4h4

Organs/Tissues associated with anti-Hist1h3a antibody

 Organ/Tissue Name  Pubmed Publications
 Eye Antibodies  >2 publications with Hist1h3a and Eye
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