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Bims/El/L Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS566334

MBS566334 is an antibody targeting Bcl2l11. Also known as BimS/EL/L monoclonal antibody (14A8). Reactive in Human, Mouse, Monkey. Host: Rat. More details

Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
BimS/EL/L (Bcl2l11), Monoclonal Antibody
Full Product Name
BimS/EL/L monoclonal antibody (14A8)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
14A8
Host
Rat
Species Reactivity
Human, Mouse, Monkey
Form/Format
Liquid. In PBS containing 10mM sodium azide
Purity/Purification
Protein G affinity purified
Specificity
Recognizes BimL, BimEL and BimS
Preparation and Storage
For long term storage, store at -20 degree C. Avoid freeze/thaw cycles
Shipping: Shipped on Blue Ice
Molecular Weight
12,954 Da
3D Structure
ModBase 3D Structure for O54918
Immunogen
Recombinant mouse BimS
Product Notes
As Bim expression is generally low in non-transfected cell lines, please take care when choosing positive controls. Examples of cell lines which express detectable levels of Bim include: B6.2.16.BW2 or KO52.DA20 (T cell lymphomas) or ABL5.8.1 (B cell lymphoma) or MCF-7s. It is generally necessary to use a high amount of lysate (e.g. 1 x 10^7 cells). When using tissue samples, it is generally necessary to use 1-5mg of total protein from an appropriate tissue source
Applications Tested/Suitable for anti-Bcl2l11 antibody
Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-Bcl2l11 antibody
FC/FACS: 0.5-1ug/ml; over expressing cell lines
ICC: 1-5ug/ml
IHC-P: 1-5ug/ml
IP: 1-2ug/ml
WB: 1-2ug/ml

Other Notes

Small volumes of anti-Bcl2l11 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for Bcl2l11. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
bcl-2-like protein 11 isoform 3
NCBI Official Synonym Full Names
BCL2-like 11 (apoptosis facilitator)
NCBI Official Symbol
Bcl2l11
NCBI Official Synonym Symbols
Bim; Bod; bcl2-L-11; 1500006F24Rik
UniProt Protein Name
Bcl-2-like protein 11
UniProt Synonym Protein Names
Bcl2-interacting mediator of cell death
Protein Family
UniProt Gene Name
Bcl2l11
UniProt Synonym Gene Names
Bim; Bcl2-L-11
UniProt Entry Name
B2L11_MOUSE
NCBI Protein Information
bcl-2-like protein 11; Bcl2 interacting mediator of cell death; bcl2-interacting mediator of cell death

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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