Mouse Anti-BGLAP Monoclonal Antibody – Cat# MBS566356

Osteocalcin (BGLAP) Antibody – Mouse Monoclonal

Name: BGLAP Antibody; Monoclonal; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS566356
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS566356

MBS566356 is an antibody targeting BGLAP. Also known as Osteocalcin (undercarboxylated) monoclonal antibody (GluOC4-5). Reactive in Human, Rat, Sheep, Rabbit, Porcine, Guinea pig, Goat, BovineDoes not cross-react with Gla-type osteocalcin. Host: Mouse. More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

Osteocalcin (BGLAP), Monoclonal Antibody

Full Product Name

Osteocalcin (undercarboxylated) monoclonal antibody (GluOC4-5)

Product Gene Name

anti-BGLAP antibody

Clonality

Monoclonal

Isotype

IgG1

Clone Number

GluOC4-5

Host

Mouse

Species Reactivity

Human, Rat, Sheep, Rabbit, Porcine, Guinea pig, Goat, Bovine
Does not cross-react with Gla-type osteocalcin

Form/Format

Lyophilized from 10mM PBS, pH7.4, containing 1% BSA. Contains no preservatives

Specificity

Recognizes human osteocalcin with decarboxylated glutamic acid residues 21 and 24

Preparation and Storage

For long term storage, store at -20 degree C
Reconstituted solution can be stored at 4 degree C for 6 months and at -20 degree C for 1 year
Shipping: Shipped on Blue Ice

Molecular Weight

10,963 Da

Chromosome Location

Chromosome: 1; NC_000001.10 (156211951..156213123). Location: 1q22

OMIM

112260

3D Structure

ModBase 3D Structure for P02818

Source Note

Obtained by fusing the mouse myeloma cells line P3U1 with splenocytes of BALB/c mouse immunized with KLH-conjugated peptide from human osteocalcin. Purified from ascites by ammonium sulfate precipitation and anion exchange chromatography

Reconstitution

Dissolve in 50ul distilled water to a final concentration of 2mg/ml. Dilute with 10mM PBS, pH7.4, containing 1% BSA and 0.1% sodium azide

Immunogen

Synthetic peptide corresponding to aa 14-30 human osteocalcin decarboxylated glutamic acid residues 21 and 24

Product Categories/Family for anti-BGLAP antibody

Cellular Analysis; Cell Organelles & Structures

Applications Tested/Suitable for anti-BGLAP antibody

ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

Application Notes for anti-BGLAP antibody

ELISA: 5-10ug/ml; colorimetric detection; use combination of clones GluOC4-5 (solid phase) and OCG3 (labelled)
IHC-P: 5-10ug/ml; Fixation by AMeX modified method
WB: 5-10ug/ml

Other Notes

Small volumes of anti-BGLAP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for BGLAP. It may not necessarily be applicable to this product.

NCBI GI #

40316933

NCBI GeneID

632

NCBI Accession #

NP_954642.1

UniProt Primary Accession #

P02818

UniProt Secondary Accession #

Q5TCK6

UniProt Related Accession #

P02818

GenBank Nucleotide #

NM_199173.4

NCBI Official Full Name

osteocalcin preproprotein

NCBI Official Synonym Full Names

bone gamma-carboxyglutamate (gla) protein

NCBI Official Symbol

BGLAP

NCBI Official Synonym Symbols

OC; BGP; OCN

UniProt Protein Name

Osteocalcin

UniProt Synonym Protein Names

Bone Gla protein; BGP; Gamma-carboxyglutamic acid-containing protein

Protein Family

Osteocalcin

UniProt Gene Name

BGLAP

UniProt Synonym Gene Names

BGP

UniProt Entry Name

OSTCN_HUMAN

NCBI Protein Information

osteocalcin; bone Gla protein; gamma-carboxyglutamic acid-containing protein; bone gamma-carboxyglutamate (gla) protein (osteocalcin)

NCBI Summary for BGLAP

This gene encodes a highly abundant bone protein secreted by osteoblasts that regulates bone remodeling and energy metabolism. The encoded protein contains a Gla (gamma carboxyglutamate) domain, which functions in binding to calcium and hydroxyapatite, the mineral component of bone. Serum osteocalcin levels may be negatively correlated with metabolic syndrome. Read-through transcription exists between this gene and the neighboring upstream gene, PMF1 (polyamine-modulated factor 1), but the encoded protein only shows sequence identity with the upstream gene product. [provided by RefSeq, Jun 2015]

UniProt Comments for BGLAP

osteocalcin: Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. Belongs to the osteocalcin/matrix Gla protein family.

Protein type: Secreted, signal peptide; Secreted; Cell development/differentiation

Chromosomal Location of Human Ortholog: 1q22

Cellular Component: Golgi apparatus; extracellular space; rough endoplasmic reticulum; dendrite; cytoplasm; perikaryon

Molecular Function: structural constituent of bone; hydroxyapatite binding; calcium ion binding; structural molecule activity

Biological Process: response to drug; response to gravity; regulation of bone resorption; response to glucocorticoid stimulus; cell aging; response to testosterone stimulus; bone mineralization; osteoblast development; odontogenesis; osteoblast differentiation; regulation of osteoclast differentiation; response to ethanol; response to vitamin D; response to vitamin K; response to estrogen stimulus; response to mechanical stimulus; response to zinc ion; response to hydroxyisoflavone; response to activity; cell adhesion; regulation of bone mineralization; skeletal development

Research Articles on BGLAP

1. Data suggest that serum level of total osteocalcin during the follicular phase is positively associated with fat-free mass in healthy premenopausal women.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-BGLAP antibody

Reference Product	PubMed Publications
RUNX2 antibody	>27 publications with BGLAP and RUNX2
BMP2 antibody	>9 publications with BGLAP and BMP2
TGFB1 antibody	>3 publications with BGLAP and TGFB1
VDR antibody	>3 publications with BGLAP and VDR
LEP antibody	>1 publications with BGLAP and LEP
PTHLH antibody	>1 publications with BGLAP and PTHLH
PTH antibody	>1 publications with BGLAP and PTH
ATF4 antibody	>1 publications with BGLAP and ATF4

Pathways associated with anti-BGLAP antibody

Products by Pathway	Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle, Mitotic Pathway antibodies	Cell Cycle, Mitotic Pathway Diagram
FGF Signaling Pathway antibodies	FGF Signaling Pathway Diagram
Glucocorticoid Receptor Regulatory Network Pathway antibodies	Glucocorticoid Receptor Regulatory Network Pathway Diagram
Interleukin-11 Signaling Pathway antibodies	Interleukin-11 Signaling Pathway Diagram
M Phase Pathway antibodies	M Phase Pathway Diagram
Mitotic Anaphase Pathway antibodies	Mitotic Anaphase Pathway Diagram
Mitotic Metaphase And Anaphase Pathway antibodies	Mitotic Metaphase And Anaphase Pathway Diagram
Mitotic Prometaphase Pathway antibodies	Mitotic Prometaphase Pathway Diagram
Notch-mediated HES/HEY Network Pathway antibodies	Notch-mediated HES/HEY Network Pathway Diagram

Diseases associated with anti-BGLAP antibody

Disease Name	Pubmed Publications
Bone Diseases Antibodies	>8 publications with BGLAP and Bone Diseases
Osteoporosis Antibodies	>5 publications with BGLAP and Osteoporosis
Neoplasms Antibodies	>4 publications with BGLAP and Neoplasms
Hemorrhage Antibodies	>1 publications with BGLAP and Hemorrhage
Pain Antibodies	>1 publications with BGLAP and Pain
Hypertension Antibodies	>1 publications with BGLAP and Hypertension
Cardiovascular Diseases Antibodies	>1 publications with BGLAP and Cardiovascular Diseases
Inflammation Antibodies	>1 publications with BGLAP and Inflammation
Necrosis Antibodies	>1 publications with BGLAP and Necrosis
Brain Injuries Antibodies	>1 publications with BGLAP and Brain Injuries

Organs/Tissues associated with anti-BGLAP antibody

Organ/Tissue Name	Pubmed Publications
Bone Antibodies	>49 publications with BGLAP and Bone
Brain Antibodies	>3 publications with BGLAP and Brain
Parathyroid Antibodies	>1 publications with BGLAP and Parathyroid
Prostate Antibodies	>1 publications with BGLAP and Prostate