HDAC2 Active Protein

Name: HDAC2 Active Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS566683
Price: 1025 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS566683

HDAC2, like the highly homologous HDAC1, is a class I HDAC first identified as a human homolog of the yeast histone deacetylase Rpd3. HDAC2 found, along with HDAC1 in the Sin3, NuRD. In contrast to class II HDACs, which repress cardiac hypertrophy, HDAC2… More details

$1025 / 0.05 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

HDAC2, Active Protein

Full Product Name

HDAC2 (full-length) (human), (recombinant)

Product Gene Name

HDAC2 active protein

Host

Insect cells

Form/Format

Liquid. In 50mM TRIS, pH8.0, 138 mM NaCl and 10% glycerol

Purity/Purification

Partially purified by single-step affinity chromatography and gel filtration

Preparation and Storage

For long term storage, store at -80 degree C
Shipping: Shipped on Dry Ice

Molecular Weight

~66kDa

OMIM

605164

3D Structure

ModBase 3D Structure for Q92769

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Source

Produced in insect cells. Produced in a baculovirus expression system

Sequence

HDAC2 from human cDNA. Sequence is identical to residues 1-582 at Genbank accession# NM_00157.2

Specific Activity

>0.5 U/ug. One U=1 pmol/min at 37 degree C, 100 uM FLUOR DE LYS - SIRT1 substrate (p53K(Ac)-382 fluorogenic substrate)

Note

The enzyme is stable on ice for the time typically required to set up an experiment (30 min-60 min) but may lose activity with prolonged storage on ice. It is recommended that thawing and dilution of the enzyme be done within as short a time as possible before start of the assay. The remaining, unused, undiluted enzyme should be refrozen quickly. For example, snap freezing in a dry/ice ethanol bath or liquid nitrogen. Freezing and storage of diluted enzyme is not recommended

Dry Ice Shipment

Extra charge fee may add to your shipping cost as dry ice is required to ship this product.

Product Categories/Family for HDAC2 active protein

Cellular Analysis; Cell Death; Stem Cells; Epigenetics; Histone Posttranslational Modifications; Proteostasis; Posttranslational Modifications; Proteolysis & Protein Degradation

Other Notes

Small volumes of HDAC2 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HDAC2 purchase

MBS566683 is an active protein targeting HDAC2. Also known as HDAC2 (full-length) (human), (recombinant). Host: Insect cells.

Additional Details

NCBI/Uniprot data below describe general gene information for HDAC2. It may not necessarily be applicable to this product.

NCBI Accession #

NM_00157.2

UniProt Primary Accession #

Q92769

UniProt Protein Name

Histone deacetylase 2

UniProt Gene Name

HDAC2

UniProt Synonym Gene Names

HD2

UniProt Entry Name

HDAC2_HUMAN

UniProt Comments for HDAC2

HDAC2: a transcriptional regulator of the histone deacetylase family, subfamily 1. Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation plays a role in epigenetic repression and transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.

Protein type: Deacetylase; Transcription, coactivator/corepressor; EC 3.5.1.98; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: nucleoplasm; heterochromatin; transcription factor complex; protein complex; Sin3 complex; ESC/E(Z) complex; nuclear chromatin; cytoplasm; replication fork; NuRD complex; nucleus

Molecular Function: deacetylase activity; NAD-dependent histone deacetylase activity (H3-K9 specific); protein binding; enzyme binding; nucleosomal DNA binding; NAD-dependent histone deacetylase activity (H3-K14 specific); sequence-specific DNA binding; heat shock protein binding; protein deacetylase activity; NAD-dependent histone deacetylase activity (H4-K16 specific); chromatin binding; histone deacetylase activity; transcription factor activity; transcription factor binding

Biological Process: establishment and/or maintenance of chromatin architecture; nerve growth factor receptor signaling pathway; positive regulation of proteolysis; positive regulation of transcription, DNA-dependent; positive regulation of collagen biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic; negative regulation of cell cycle; cardiac muscle cell development; negative regulation of gene expression, epigenetic; negative regulation of cardiac muscle cell proliferation; positive regulation of cell proliferation; dendrite development; circadian regulation of gene expression; positive regulation of oligodendrocyte differentiation; response to drug; transcription, DNA-dependent; negative regulation of transcription factor activity; hippocampus development; histone deacetylation; regulation of protein kinase B signaling cascade; response to cocaine; odontogenesis of dentine-containing teeth; chromatin remodeling; ATP-dependent chromatin remodeling; negative regulation of MHC class II biosynthetic process; maintenance of chromatin silencing; epidermal cell differentiation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; gene expression; embryonic digit morphogenesis; negative regulation of transcription, DNA-dependent; blood coagulation; negative regulation of apoptosis

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.