Cd11a (Activitor) Antibody – Mouse Monoclonal

Integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide), also known as ITGAL, is a Human gene which functions in the immune system. It is involved in cellular adhesion and costimulatory signaling. ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 is part of the family of leukocyte integrins that are recognised by their common beta-chains (CD18). LFA-1 also has a distinct alpha-chain (CD11a). LFA-1 is expressed on lymphocytes, monocytes and granulocytes; it has been shown that LFA-1 contributes to the adhesion reaction of these cells. Blocking experiments with anti-LFA monoclonal antibodies demonstRated that LFA-1 inhibits the adhesion step effector and target cells in cytotoxic T lymphocyte, natural killer and lectin dependent cytotoxicity. LFA-1 is involved in recruitment to the site of infection. It binds to ICAM-1 on antigen-presenting cells and functions as an adhesion molecule. LFA-1 is the first to bind T-cells to antigen-presenting cells and initially binds weakly. A signal from the T-cell receptor and/or the cytokine receptor changes the conformation and prolongs the cell contact, allowing the T-cell to prolifeRate. A unique antibody NKI(SPV)-L16, reacting with the a-chain of the Human leukocyte function-associated Ag- 1 (LFA- 1) has been made available. This antibody stimulates homotypic cell-cell interactions in a manner very similar to 12-0-tetradecanoyl-phorbol1-3 -acetate (TPA), in contrast to other anti-LFA-1 mAb which inhibit cell aggregation. Immunoprecipitation and enzyme digestion studies revealed that NKI-L16 recognizes a unique epitope on the cy-chain of LFA-1,most likely situated close to the transmembrane segment of the molecule. It is hypothesized that NKI-L16 or TPA can cause the LFA-1 molecule to convert from an inactive to an active configuRation, thereby permitting binding of LFA-1 1 to its natural ligand. This mAb directed, in contrast to other anti-LFA-1 antibodies, stimulates Rather than inhibits homotypic cell aggregate formation, but does not affect cell prolifeRation.

Keizer GD, Visser W, Vliem M, Figdor CG. A monoclonal antibody (NKI-L16) directed anti a unique epitope on the alpha chain of Human leukocyte function-associated antigen 1 induces homotypic cell-cell interactions. J. Immunol. 140: 1393-1400, 1988. Van Kooyk Y, Weder P, Hogervorst F, Verhoeven AJ, van Seventer G, te Velde AA, Borst J, Keizer GD, Figdor CG. Activation of LFA-1 through a Ca2(+)-dependent epitope stimulates lymphocyte adhesion. J Cell Biol. 112: 345-54, 1991. Van Kooyk Y, van de Wiel-van Kemenade P, Weder P, Kuijpers TW, Figdor CG. Enhancement of LFA-1-mediated cell adhesion by triggering through CD2 or CD3 on T lymphocytes. Nature. 342:811-813, 1989.

Small volumes of anti-CD11a (activitor) antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS570272 is an antibody targeting CD11a (activitor). Also known as Mouse anti CD11a (activitor). Reactive in Human. Host: Mouse (Balb/c).