Ac-GLY-BoroPro Inhibitor

Solid. Powder

98.00%

Store at -20 degree C for 3 years powder
-80 degree C for 2 years in solvent

214.03

DMSO: 50mg/mL (233.61mM)
(<1mg/ml refers to the product slightly soluble or insoluble)

C8H15BN2O4

O=C(N1[C@@H](B(O)O)CCC1)CNC(C)=O

886992-99-0

FAP(ki): ic50 23nM (ki)

Ki values for inhibition of proteases by Ac-Gly-BoroPro are determined using the method of progress curves for analysis of tight-binding competitive inhibitors. Various concentrations of Ac-Gly-BoroPro are reacted with FAP (1.0nM) and DPP-4 (0.1nM) in the presence of Ala-Pro-AFC (500uM for FAP; 100uM for DPP-4), and time-dependent inhibition of each protease is monitored. Reactions contained inhibitor concentrations at least 20-fold greater than protease concentrations, such that the protease-inhibitor complex does not significantly deplete the free inhibitor.