AVE0991 Inhibitor – AVE 0991

Name: AVE0991 Inhibitor; AVE 0991; MyBioSource
Brand: MyBioSource
SKU: MBS5764315
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS5764315

AVE 0991 is a small molecular and orally active agonist of angiotensin-(1-7) receptor (IC50: 21nM). More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

AVE 0991 (AVE0991), Inhibitor

Full Product Name

AVE 0991

Product Gene Name

AVE0991, inhibitor

Form/Format

Solid. Powder

Purity/Purification

98.00%

Preparation and Storage

Store at -20 degree C for 3 years powder
-80 degree C for 2 years in solvent

Molecular Weight

580.72

Solubility

DMSO: 100mg/mL (172.20mM)
Water: Insoluble
(<1mg/ml refers to the product slightly soluble or insoluble)

Formula

C29H32N4O5S2

SMILES

CCNC(NS(c(sc(CC(C)C)c1)c1-c1ccc(Cn2c(-c3ccccc3)nc(OC)c2C=O)cc1)(=O)=O)=O

CAS Number

304462-19-9

Cell Research

COS cells and CHO cells are stably transfected with rat Mas cDNA driven by a cytomegalovirus promoter and selected by neomycin. 125I-Ang-(1-7) (0.5x10^-9 mol/L) is incubated in 24-well plates for 60 minutes at 4 degree C in 0.3mL of serum-free medium (DMEM) supplemented with 0.2% BSA, 0.005% bacitracin, 0.1 mol/L PMSF, and 0.5 mol/L orthophenanthroline with Mas-transfected COS cells in the presence or absence of AVE 0991 (AVE, 10-10 to -5 mol/L). After 2 ishes with ice-cold serum-free DMEM, cells are disrupted with 0.1% Triton X-100. Bound radioactivity is measured in a gamma counter. Binding of rhodamine-Ang-(1-7) in Mas-transfected CHO cells is performed under similar conditions using 2x10^-9 mol/L rhodamine-labeled-Ang-(1-7) in the presence or absence of AVE (10^-6 mol/L), CV11974 (10^-6 mol/L), or PD123319 (10^-6 mol/L). NSB is determined in the presence of 10-6 mol/L Ang-(1-7) [1].

Kinase Assay

Briefly, 100 ug of membranes from primary cultured bovine aortic endothelial cells (BAECs, passage 1) are incubated in a total volume of 200uL for 45 minutes at 25 degree C in HEPES-buffered saline (10mM HEPES, 0.1 M NaCl, 5mM MgCl2) containing 0.2% BSA and protease inhibitor cocktail Complete. Saturable binding of [125I]-Ang-(1-7) is calculated by subtracting nonspecific binding (40% to 50%), determined in the presence of 10uM unlabeled Ang-(1-7) from total binding. Competition experiments with increasing concentrations of AVE 0991 and unlabeled Ang-(1-7) are performed in the presence of 10nM [125I]-Ang-(1-7). Assays are terminated by vacuum filtration (<=15mM Hg) over filters (0.65uM, Opak 96-well plates) presoaked with 1% BSA. The filters are washed 3 times with each 100uL of PBS (50mM, NaHPO4 and 0.15M NaCl, pH 7.2). Radioactivity on dried filters is quantified with a gamma counter [1].

Product Categories/Family for AVE0991, inhibitor

Endocrinology/Hormones; RAAS

Product References and Citations for AVE0991, inhibitor

Wiemer G, et al. AVE 0991, a nonpeptide mimic of the effects of angiotensin-(1-7) on the endothelium. Hypertension. 2002 Dec;40(6):847-52.
Wiemer G, et al. AVE 0991, a nonpeptide mimic of the effects of angiotensin-(1-7) on the endothelium. Hypertension. 2002 Dec;40(6):847-52.
Pinheiro SV, et al. Nonpeptide AVE 0991 is an angiotensin-(1-7) receptor Mas agonist in the mouse kidney. Hypertension. 2004 Oct;44(4):490-6.
Pinheiro SV, et al. Nonpeptide AVE 0991 is an angiotensin-(1-7) receptor Mas agonist in the mouse kidney. Hypertension. 2004 Oct;44(4):490-6.
Ferreira AJ, et al. The nonpeptide angiotensin-(1-7) receptor Mas agonist AVE-0991 attenuates heart failure induced by myocardial infarction. Am J Physiol Heart Circ Physiol. 2007 Feb;292(2):H1113-9.
Ferreira AJ, et al. The nonpeptide angiotensin-(1-7) receptor Mas agonist AVE-0991 attenuates heart failure induced by myocardial infarction. Am J Physiol Heart Circ Physiol. 2007 Feb;292(2):H1113-9.

Other Notes

Small volumes of AVE0991, inhibitor vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for AVE0991 purchase

MBS5764315 is an inhibitor targeting AVE0991. Also known as AVE 0991.