C9 Neoantigen Antibody – Mouse Monoclonal

The monoclonal antibody WU13-15 recognizes a neoepitope on the 61 kDa complement component C9, integrated in the terminal complement complex (TCC). The complement system is an ancient proinflammatory and microbial destruction system, that may be considered part of both the innate and adaptive immune systems. It consists of the classical, alternative, and lectin-binding pathways. Each pathway is triggered in a distinct manner, yet all deposit C3 fragments on a target and engage a common terminal sequence called TCC or the "membrane attack complex" (MAC). In contrast to the activation pathways, which require enzymatic cleavage for activation, the terminal pathway relies on conformational changes induced by binding of the different subunits. TCC is composed of a complex of four complement proteins (C5b, C6, C7, and C8) which bind to the outer surface of the target plasma membrane, and many copies of a fifth protein (C9) that hookup to one another, forming a ring in the membrane. The ring structure formed by C9 is a pore in the membrane that allows free diffusion of molecules in and out of the cell. If enough pores form, the cell is no longer able to survive. The membrane attack complex is initiated when the complement protein, C5 convertase, cleaves C5 into C5a and C5b. Binding of C6 facilitates binding of C7 which alters the conformation of the complex. After binding of C8, a variable number of C9 molecules associate with the C5b678 complex, together constituting TCC. The formation of TCC causes lysis of cells or can trigger a variety of cellular metabolic pathways resulting in the synthesis and release of inflammatory mediators. TCC contains neoantigens that are absent in the individual native C9 proteins. Neoantigens are present both in the membranebound (MAC) and the fluid phase (SC5b-9) complex. TCC is present in normal human plasma and increases upon complement activation.

1. Breitner-Ruddock, S et al; Heterogeneity in the complement-dependent bacteriolysis within the species of Borrelia burgdorferi. Med Microbiol Immunol 1997, 185: 253. 2. Kraiczy, P et al; Borreliacidal activity of early Lyme disease sera against complement-resistant Borrelia afzelii FEM1 wild-type and an OpsC-lacking FEM1 variant. J Med Microbiol 2000, 49: 917. 3. Meri, T et al; Onchocerca volvulus microfilariae avoid complement attack by direct binding of factor H. J Infect Dis 2002, 185: 1786. 4. Würzner, R et al; Blood dendritic cells carry terminal complement complexes on their cell surface as detected by newly developed neoepitope-specific monoclonal antibodies. Immunol 1991, 74: 132.

Small volumes of anti-C9 neoantigen antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS584814 is an antibody targeting C9 neoantigen. Also known as C9 neoantigen, Human, mAb WU13-15. Reactive in Human. Host: Mouse.