PDS1 Antibody – Mouse Monoclonal

Function: Regulatory protein, which plays a central role in chromosome stability. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESP1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESP1. Ref.6

Subunit structure: Interacts with the caspase-like ESP1, and prevents its protease activity probably by covering its active site. Interacts with CDC20. Ref.6 Ref.7

Subcellular location: Cytoplasm. Nucleus.

Domain: The N-terminal destruction box (D-box) acts as a recognition signal for degradation via the ubiquitin-proteasome pathway

By similarity.

Post-translational modification: Phosphorylated by CDC28. The phosphorylation may be important for ESP1 localization to the nucleus. Ref.8Ubiquitinated by the anaphase promoting complex (APC) at the onset of anaphase, conducting to its degradation. Ref.5

Sequence similarities: Belongs to the securin family.