Mouse Anti-DNMT3A Monoclonal Antibody – Cat# MBS601364

Western Blot analysis for Dnmt3a using MBS601364 at 2ug/ml against 10ug of 293 cell lysate transfected with either mouse Dnmt3a (lane 1) or mouse Dnmt3b (lane 2).

DNA Methyltransferase 3a (DNMT3A) Antibody – Mouse Monoclonal

Name: DNMT3A Antibody; Monoclonal; Mouse; Human/Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS601364
Price: 820 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS601364

$820 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

DNA Methyltransferase 3A (Dnmt3a), Monoclonal Antibody

Full Product Name

DNA Methyltransferase 3A (Dnmt 3A)

Product Gene Name

anti-Dnmt3a antibody

Product Synonym Names

Anti -DNA Methyltransferase 3A (Dnmt 3A)

Clonality

Monoclonal

Isotype

IgG1

Clone Number

4H82

Host

Mouse

Species Reactivity

Human, Mouse

Form/Format

Supplied as a liquid in PBS, 0.02% sodium azide.

Purity/Purification

Purified
Purified

Specificity

Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Recently, it has been shown that naturally occurring mutations of Dnmt3b gene occurs in patients with a rare autosomal recessive disorder, termed ICF (immunodeficiency, centromeric instability, and facial anomalies) syndrome.

Preparation and Storage

May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

101,669 Da

Chromosome Location

Chromosome: 6; NC_005105.2 (26802612..26878878). Location: 6q14

3D Structure

ModBase 3D Structure for Q1LZ53

Immunogen

Bacteria expressed HIS-tag recombinant mouse Dnmt3a.

Product Categories/Family for anti-Dnmt3a antibody

Antibodies; Abs to Enzymes; Methyltransferase

Applications Tested/Suitable for anti-Dnmt3a antibody

Western Blot (WB)

Application Notes for anti-Dnmt3a antibody

Suitable for use in Western Blot.
Dilution: Western Blot: 2ug/ml
Western Blot: 2ug/ml

Other Notes

Small volumes of anti-Dnmt3a antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Dnmt3a purchase

MBS601364 is an antibody targeting Dnmt3a. Also known as DNA Methyltransferase 3A (Dnmt 3A). Reactive in Human, Mouse. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Dnmt3a. It may not necessarily be applicable to this product.

NCBI GI #

51556265

NCBI GeneID

444984

NCBI Accession #

NP_001003957.1

UniProt Primary Accession #

Q1LZ53

UniProt Secondary Accession #

Q1LZ52

UniProt Related Accession #

Q1LZ53; Q5FVL1

GenBank Nucleotide #

NM_001003957.1

NCBI Official Full Name

DNA (cytosine-5)-methyltransferase 3A isoform 2

NCBI Official Synonym Full Names

DNA (cytosine-5-)-methyltransferase 3 alpha

NCBI Official Symbol

Dnmt3a

NCBI Official Synonym Symbols

MGC109195

UniProt Protein Name

DNA (cytosine-5)-methyltransferase 3A

Protein Family

DNA

UniProt Gene Name

Dnmt3a

UniProt Entry Name

DNM3A_RAT

NCBI Protein Information

DNA (cytosine-5)-methyltransferase 3A; DNA (cytosine-5)-methyltransferase 3A; DNA methyltransferase 3A

NCBI Summary for Dnmt3a

member of a family of DNA methyltransferases [RGD]

UniProt Comments for Dnmt3a

DNMT3A: a DNA methyltransferase required for genome wide de novo methylation and essential for development. DNA methylation is coordinated with methylation of histones. Prefers CpG methylation to CpA, CpT, and CpC methylation. Methylates CpG sites at a rate much slower than DNMT1, but greater than DNMT3b. Methylation is coordinated with methylation of histones. Binds the ZNF238 transcriptional repressor. Interacts with SETDB1 and DNMT1. Can co-localize with heterochromatin protein (HP1 ) and methyl-CpG binding protein (MeCBP). Associates with HDAC1 through its ADD-type zinc-finger. Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung.

Protein type: Methyltransferase, DNA; Amino Acid Metabolism - cysteine and methionine; Methyltransferase; EC 2.1.1.37

Cellular Component: nucleoplasm; XY body; heterochromatin; euchromatin; nuclear matrix; cytoplasm; nuclear heterochromatin; nucleus; chromosome, pericentric region

Molecular Function: DNA (cytosine-5-)-methyltransferase activity; identical protein binding; DNA binding; DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates; unmethylated CpG binding; metal ion binding; chromatin binding

Biological Process: response to nutrient levels; response to drug; genetic imprinting; transcription, DNA-dependent; response to toxin; DNA methylation during gametogenesis; S-adenosylhomocysteine metabolic process; negative regulation of transcription from RNA polymerase II promoter; response to cocaine; response to estradiol stimulus; response to vitamin A; neuron differentiation; cytosine methylation within a CG sequence; response to ethanol; methylation-dependent chromatin silencing; response to lead ion; DNA methylation; S-adenosylmethioninamine metabolic process; spermatogenesis; response to ionizing radiation; mitotic cell cycle; DNA methylation during embryonic development; DNA methylation on cytosine; aging

Research Articles on Dnmt3a

1. Suggest that DNMT1 and DNMT3a protein overexpression may be responsible for aberrant DNA methylation of cell adhesion genes in chemically induced lung neoplasms.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Dnmt3a antibody

Reference Product	PubMed Publications
Cdkn1a antibody	>10 publications with Dnmt3a and Cdkn1a

Pathways associated with anti-Dnmt3a antibody

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One Carbon Metabolism Pathway antibodies	One Carbon Metabolism Pathway Diagram

Diseases associated with anti-Dnmt3a antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>132 publications with Dnmt3a and Neoplasms
Carcinoma Antibodies	>53 publications with Dnmt3a and Carcinoma
Leukemia Antibodies	>29 publications with Dnmt3a and Leukemia
Adenocarcinoma Antibodies	>22 publications with Dnmt3a and Adenocarcinoma
Skin Diseases Antibodies	>20 publications with Dnmt3a and Skin Diseases
Male Urogenital Diseases Antibodies	>20 publications with Dnmt3a and Male Urogenital Diseases
Liver Diseases Antibodies	>19 publications with Dnmt3a and Liver Diseases
Breast Neoplasms Antibodies	>18 publications with Dnmt3a and Breast Neoplasms
Female Urogenital Diseases Antibodies	>17 publications with Dnmt3a and Female Urogenital Diseases
Liver Neoplasms Antibodies	>17 publications with Dnmt3a and Liver Neoplasms

Organs/Tissues associated with anti-Dnmt3a antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>34 publications with Dnmt3a and Brain
Embryonic Tissue Antibodies	>24 publications with Dnmt3a and Embryonic Tissue
Lung Antibodies	>16 publications with Dnmt3a and Lung
Prostate Antibodies	>12 publications with Dnmt3a and Prostate
Kidney Antibodies	>11 publications with Dnmt3a and Kidney
Ovary Antibodies	>11 publications with Dnmt3a and Ovary
Colon Antibodies	>9 publications with Dnmt3a and Colon
Thymus Antibodies	>5 publications with Dnmt3a and Thymus
Placenta Antibodies	>4 publications with Dnmt3a and Placenta
Heart Antibodies	>3 publications with Dnmt3a and Heart