Mouse Anti-POU4F1 Monoclonal Antibody – Cat# MBS601428

Staining of monkey retina. Localization of Brn-3a in a subset of nuclei in the gangion cell layer.

POU4F1 Antibody – Mouse Monoclonal

Name: POU4F1 Antibody; Monoclonal; Mouse; Chicken-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS601428
Price: 1095 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS601428

Brn3a (transcription factor 1 (brain specific homeobox/POU domain protein 3A) POU4F1, POU domain, class 4, RDC-1) is a transcription factor specifically expressed in cells of the developing mammalian nervous system. More details

$1095 / 1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Brn3A (Pou4f1), Monoclonal Antibody

Full Product Name

Brn3A (Pou Domain Transcription Factor)

Product Gene Name

anti-Pou4f1 antibody

Product Synonym Names

Anti -Brn3A (Pou Domain Transcription Factor)

Clonality

Monoclonal

Isotype

IgG1

Clone Number

2Q24

Host

Mouse

Species Reactivity

Chicken, Monkey

Form/Format

Supplied as a liquid. No preservative added.

Purity/Purification

Supernatant
Culture supernatant

Specificity

Brn-3a. Shows no reactivity to Brn-3b or Brn-3c by Western blot. Showed no reactivity to Brn-3a knock-out mice. Species Reactivity: Monkey and chicken

Preparation and Storage

May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

42,767 Da

Chromosome Location

chromosome: 14; Location: 14; 14 E1-E3

3D Structure

ModBase 3D Structure for P17208

Immunogen

aa186-224 of Brn-3a fused to the T7 gene 10 protein (1).

Product Categories/Family for anti-Pou4f1 antibody

Antibodies; Abs to Proteins

Applications Tested/Suitable for anti-Pou4f1 antibody

Western Blot (WB), Immunohistochemistry (IHC)

Application Notes for anti-Pou4f1 antibody

Suitable for use in Immunohistochemistry, Western Blot.
Dilution: Immunohistochemistry: 1:5-1:125
Western blot: 1:50-1:100

Other Notes

Small volumes of anti-Pou4f1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Pou4f1 purchase

MBS601428 is an antibody targeting Pou4f1. Also known as Brn3A (Pou Domain Transcription Factor). Reactive in Chicken, Monkey. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Pou4f1. It may not necessarily be applicable to this product.

NCBI GI #

51921650

NCBI GeneID

18996

UniProt Primary Accession #

P17208

UniProt Secondary Accession #

E9QPT6

UniProt Related Accession #

P17208; Q64FM2; Q78NA5; Q80ZA2

NCBI Official Full Name

Brn3a

NCBI Official Synonym Full Names

POU domain, class 4, transcription factor 1

NCBI Official Symbol

Pou4f1

NCBI Official Synonym Symbols

Brn3; Brn-3; Brn3a; Brn3.0; Brn-3.0; E130119J07Rik; Pou4f1

UniProt Protein Name

POU domain, class 4, transcription factor 1

UniProt Synonym Protein Names

Brain-specific homeobox/POU domain protein 3A; Brain-3A; Brn-3A; Brn-3.0

Protein Family

POU domain, class 4, transcription factor

UniProt Gene Name

Pou4f1

UniProt Synonym Gene Names

Brn-3; Brn3; Brn3a

UniProt Entry Name

PO4F1_MOUSE

NCBI Protein Information

POU domain, class 4, transcription factor 1; brn-3A; brain-3A; brain-specific homeobox/POU domain protein 3A

UniProt Comments for Pou4f1

POU4F1: Probable transcription factor which may play a role in the regulation of specific gene expression within a subset of neuronal lineages. May play a role in determining or maintaining the identities of a small subset of visual system neurons. Belongs to the POU transcription factor family. Class- 4 subfamily.

Protein type: DNA-binding; Cell development/differentiation; Transcription factor

Cellular Component: neuron projection; nuclear chromatin; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; DNA binding; sequence-specific DNA binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; nervous system development; trigeminal nerve development; central nervous system neuron differentiation; transcription, DNA-dependent; positive regulation of apoptosis; multicellular organismal development; peripheral nervous system neuron differentiation; negative regulation of transcription from RNA polymerase II promoter; habenula development; regulation of transcription from RNA polymerase II promoter; cell migration in hindbrain; neuron differentiation; peripheral nervous system neuron development; proprioception during equilibrioception; regulation of neurogenesis; regulation of transcription, DNA-dependent; suckling behavior; neuron fate specification; mesoderm development; positive regulation of transcription from RNA polymerase II promoter; sensory system development; negative regulation of neuron apoptosis

Research Articles on Pou4f1

1. data define AR as a direct Brn-3a interactor and verify a simple interacting protein prediction methodology that is likely to be useful for many other proteins
2. Data show that Brn3a acts upstream of the Runx factors, which then repress TrkB expression to allow establishment of the non-overlapping Trk receptor profiles and correct terminally differentiated phenotypes.
3. Brn3a regulates the transition from neurogenesis to terminal differentiation and represses non-neural gene expression in the trigeminal ganglion.
4. these findings begin to define a gene regulatory pathway for habenula development in mammals, and the roles of Nurr1 and Brn3a therein.
5. Brn-3a deficiency affects the number and cell size of CGRP- and calcium-binding protein-containing neurons.
6. Loss of Brn3a leads to an increase in the ratio of bistratified to monostratified RGCs, with only modest RGC loss and little effect on central projections.
7. Brn3a represses differences in gene expression between sensory neurons generated at different axial levels, and regulates developmental gene expression; functionally similar populations of neurons are generated from different embryological substrates.
8. results suggest that co-factors binding to p73 C terminus facilitate activation on Bax but not p21 CIP1/Waf1 promoter and that Brn-3a modulates this interaction
9. Data suggest that Brn-3a and b POU factors may play an important role in the development and maintenance of critical cell types and structures within the heart, in part via developmental regulation of myocardial Hsp27 expression.
10. Data show that Pou4f1 is regionally enriched in the Brainstem (Pons and Medulla) of mouse brain.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-Pou4f1 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>25 publications with Pou4f1 and Neoplasms
Nervous System Diseases Antibodies	>16 publications with Pou4f1 and Nervous System Diseases
Uterine Cervical Neoplasms Antibodies	>7 publications with Pou4f1 and Uterine Cervical Neoplasms
Brain Diseases Antibodies	>4 publications with Pou4f1 and Brain Diseases
Pain Antibodies	>4 publications with Pou4f1 and Pain
Cervical Intraepithelial Neoplasia Antibodies	>4 publications with Pou4f1 and Cervical Intraepithelial Neoplasia
Leukemia, Myeloid, Acute Antibodies	>3 publications with Pou4f1 and Leukemia, Myeloid, Acute
Disease Models, Animal Antibodies	>3 publications with Pou4f1 and Disease Models, Animal
Nerve Degeneration Antibodies	>1 publications with Pou4f1 and Nerve Degeneration
Craniofacial Abnormalities Antibodies	>1 publications with Pou4f1 and Craniofacial Abnormalities

Organs/Tissues associated with anti-Pou4f1 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>24 publications with Pou4f1 and Brain
Eye Antibodies	>21 publications with Pou4f1 and Eye
Dorsal Root Ganglion Antibodies	>17 publications with Pou4f1 and Dorsal Root Ganglion
Embryonic Tissue Antibodies	>15 publications with Pou4f1 and Embryonic Tissue
Spinal Cord Antibodies	>8 publications with Pou4f1 and Spinal Cord
Liver Antibodies	>4 publications with Pou4f1 and Liver
Testis Antibodies	>1 publications with Pou4f1 and Testis