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Vaspin Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS601670

Vaspin (visceral adipose-specific SERPIN), a newly identified adipokine, which is a member of serine protease inhibitor family. Vaspin is also a unique insulin sensitizing adipocytokine in obesity. A recent publication indicates that induction of human vaspin… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Product Name
Vaspin, Monoclonal Antibody
Full Product Name
Vaspin (Visceral Adipose-Specific SERPIN, SERPINA12, Serine Proteinase Inhibitor-clade A12)
Product Synonym Names
Anti -Vaspin (Visceral Adipose-Specific SERPIN, SERPINA12, Serine Proteinase Inhibitor-clade A12)
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
8G9
Host
Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 10% glycerol.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Concentration
1 mg/mL (lot specific)
Specificity
Recognizes human Vaspin at ~47kD. Species Crossreactivity: rat
Preparation and Storage
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant corresponding to aa21-414 of human Vaspin, expressed in E. coli .
Hybridoma
Sp2/0 myeloma cells with spleen cells from Balb/c mice.
Applications Tested/Suitable for anti-Vaspin antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-Vaspin antibody
Dilution: Western Blot (cell lysate): 1:1000-1:3000

Optimal dilutions to be determined by the researcher.

Western Blot (WB) of anti-Vaspin antibody

Western Blot analysis: Rat serum (15ug) was resolved by SDS-PAGE, transferred to NC membrane and probed with MBS601670 (1:2000). Proteins were visualized using an IgG (HRP) goat anti-mouse and an ECL detection system.
Mouse Anti-Vaspin Monoclonal Antibody – Cat# MBS601670

Other Notes

Small volumes of anti-Vaspin antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Vaspin purchase

MBS601670 is an antibody targeting Vaspin. Also known as Vaspin (Visceral Adipose-Specific SERPIN, SERPINA12, Serine Proteinase Inhibitor-clade A12). Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Vaspin. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
NCBI Official Full Name
visceral adipose tissue specific SERPIN
Protein Family

UniProt Comments for Vaspin

SERPINA12: May modulate insulin action conceivably only in the presence of its yet undefined target proteases in white adipose tissues. Belongs to the serpin family.

Protein type: Secreted, signal peptide; Secreted; Inhibitor

Cellular Component: extracellular space; plasma membrane

Molecular Function: serine-type endopeptidase inhibitor activity

Biological Process: positive regulation of phosphoinositide 3-kinase cascade; negative regulation of lipid biosynthetic process; negative regulation of gluconeogenesis; glucose metabolic process; positive regulation of insulin receptor signaling pathway
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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