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Integrin Alpha1l Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS601860

MBS601860 is an antibody targeting Integrin alpha1L. Reactive in Human. Host: Mouse. More details

$1040 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Integrin alpha1L, Monoclonal Antibody
Full Product Name
Integrin alpha1L
Product Synonym Names
Anti -Integrin alpha1L
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2Q1420
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.6, 0.1% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A Sepharose chromatography.
Specificity
Recognizes the I domain (Val151-Ala364) of human a1 integrin (CD49a). Precipitates proteins of Mr 180kD, pI 5.9-6.0 (alpha chain) and Mr 115kD, pI 4.5-5.0 (beta chain) from human lymphocyte lysates. Does not recognize rat recombinant I domain of integrin a1.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Human Integrin alpha1L

Product Categories/Family for anti-Integrin alpha1L antibody

Antibodies; Abs to Integrin
Applications Tested/Suitable for anti-Integrin alpha1L antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-Integrin alpha1L antibody
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation, Flow Cytometry and Inhibition assays.
Dilution: ELISA: 0.5ug/ml
Western Blot: 2ug/ml
Immunohistochemistry: 2ug/ml with paraformaldehyde fixation.
Immunoprecipitation: 1ug/ml
Flow Cytometry: 2ug/ml
Inhibition Assay: 1-5ug/ml. Using activated human lymphocyte binding to laminin, collagen IV and fibronectin.

Testing Data of anti-Integrin alpha1L antibody

Confocal fluorescent analysis of A431 and HEK293 cells using a 1:100 dilution of MBS601860, Integrin alpha1 and a Donkey Anti-Mouse IgG secondary antibody conjugated to Cy3 (Red). Actin filaments have been labeled with Alexa Fluor® 488 dye (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the membrane
Mouse Anti-Integrin Alpha1l Monoclonal Antibody – Cat# MBS601860

Testing Data of anti-Integrin alpha1L antibody

Huvec (non-reducing) cell lysate was probed with MBS601860, Integrin alpha1 (1:1000 dilution). Proteins were visualized using a goat anti-mouse IgG secondary antibody conjugated to HRP and a chemiluminescence detection system.|Arrow indicates target (~110kD)
Mouse Anti-Integrin Alpha1l Monoclonal Antibody, Testing Data – Cat# MBS601860

Other Notes

Small volumes of anti-Integrin alpha1L antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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