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Kinetochore Associated, Homolog (ZW10) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS601959

MBS601959 is an antibody targeting ZW10. Also known as ZW10, Kinetochore Associated, Homolog (Drosophila) (HZW10, KNTC1AP, MGC149821). Reactive in Human, Mouse, Rat. Host: Mouse. More details

$610 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ZW10, Kinetochore Associated, Homolog, Monoclonal Antibody
Full Product Name
ZW10, Kinetochore Associated, Homolog (Drosophila) (HZW10, KNTC1AP, MGC149821)
Product Gene Name
anti-ZW10 antibody
Product Synonym Names
Anti -ZW10, Kinetochore Associated, Homolog (Drosophila) (HZW10, KNTC1AP, MGC149821)
Clonality
Monoclonal
Isotype
IgG1
Clone Number
8C379
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Form/Format
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Concentration
0.1mg/ml (lot specific)
Specificity
Recognizes human ZW10, Kinetochore Associated, Homolog (Drosophila). Species Crossreactivity: mouse and rat.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Chromosome Location
Chromosome: 11; NC_000011.9 (113603909..113644425, complement). Location: 11q23.2
OMIM
603954
3D Structure
ModBase 3D Structure for O43264
Immunogen
Partial sequence of recombinant full-length protein to human ZW10, Kinetochore Associated, Homolog (Drosophila)

Product Categories/Family for anti-ZW10 antibody

Antibodies; Abs to Proteins
Applications Tested/Suitable for anti-ZW10 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-ZW10 antibody
Suitable for use in Western Blot, Immunoprecipitation, Immunofluorescence and Immunocytochemistry.
Dilution: Western Blot: 0.2-2ug/ml
Immunoprecipitation: 100-500ug/sample
Immunocytochemistry (IF): 2-100ug/ml

Western Blot (WB) of anti-ZW10 antibody

Detection of ZW10 by Western blot. Samples: Whole cell lysate from human HeLa (H, 25ug) , mouse NIH3T3 (M, 25ug) and rat F2408 (R, 25ug) cells using MBS601959. Predicted molecular weight: 88kD
Mouse Anti-ZW10 Monoclonal Antibody – Cat# MBS601959

Immunoprecipitation (IP) of anti-ZW10 antibody

Immunoprecipitation: RIPA lysate of HeLa cells was incubated with MBS601959.
Mouse Anti-ZW10 Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS601959

Immunostaining (IS) of anti-ZW10 antibody

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained using MBS601959.
Mouse Anti-ZW10 Monoclonal Antibody, Immunostaining (IS) – Cat# MBS601959

Testing Data of anti-ZW10 antibody

Mouse Anti-ZW10 Monoclonal Antibody, Testing Data – Cat# MBS601959

Other Notes

Small volumes of anti-ZW10 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ZW10. It may not necessarily be applicable to this product.

NCBI GI #
119587633
NCBI GeneID
9183
UniProt Primary Accession #
O43264
UniProt Related Accession #
O43264
NCBI Official Full Name
ZW10, kinetochore associated, homolog (Drosophila)
NCBI Official Synonym Full Names
ZW10, kinetochore associated, homolog (Drosophila)
NCBI Official Symbol
ZW10
NCBI Official Synonym Symbols
HZW10; KNTC1AP; MGC149821
UniProt Protein Name
Centromere/kinetochore protein zw10 homolog
Protein Family
Centromere/kinetochore protein
UniProt Gene Name
ZW10
UniProt Entry Name
ZW10_HUMAN
NCBI Protein Information
centromere/kinetochore protein zw10 homolog; OTTHUMP00000238248; OTTHUMP00000238249; zeste white 10 homolog; ZW10 homolog, centromere/kinetochore protein

NCBI Summary for ZW10

This gene encodes a protein that is one of many involved in mechanisms to ensure proper chromosome segregation during cell division. The encoded protein binds to centromeres during the prophase, metaphase, and early anaphase cell division stages and to kinetochore microtubules during metaphase. [provided by RefSeq]
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UniProt Comments for ZW10

ZW10: Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Involved in regulation of membrane traffic between the Golgi and the endoplasmic reticulum. Belongs to the ZW10 family.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 11q23.2

Cellular Component: kinetochore microtubule; kinetochore; spindle pole; endoplasmic reticulum membrane; membrane; endoplasmic reticulum; cytoplasm; cytosol; nucleus

Molecular Function: centromeric DNA binding; protein binding

Biological Process: meiosis; ER to Golgi vesicle-mediated transport; protein transport; mitotic sister chromatid segregation; establishment of mitotic spindle orientation; cell division; mitotic cell cycle checkpoint; regulation of exit from mitosis; protein complex assembly; mitotic cell cycle; mitotic metaphase plate congression; Golgi organization and biogenesis
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Research Articles on ZW10

1. Results together suggest that NAG links between p31 and ZW10-RINT-1 and is involved in Golgi-to-ER transport.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ZW10 antibody

 Reference Product  PubMed Publications
 ZWINT antibody  >11 publications with ZW10 and ZWINT
 RINT1 antibody  >6 publications with ZW10 and RINT1

Pathways associated with anti-ZW10 antibody

 Products by Pathway  Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram
 M Phase Pathway antibodies  M Phase Pathway Diagram
 Mitotic M-M/G1 Phases Pathway antibodies  Mitotic M-M/G1 Phases Pathway Diagram
 Mitotic Prometaphase Pathway antibodies  Mitotic Prometaphase Pathway Diagram

Diseases associated with anti-ZW10 antibody

 Disease Name  Pubmed Publications
 Melanoma Antibodies  >1 publications with ZW10 and Melanoma
 Neoplasms Antibodies  >1 publications with ZW10 and Neoplasms

Organs/Tissues associated with anti-ZW10 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >5 publications with ZW10 and Brain
 Embryonic Tissue Antibodies  >2 publications with ZW10 and Embryonic Tissue
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