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Class 1 Antigen B27,b44,b47(x-Reactive) (HLA) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS603025

MBS603025 is an antibody targeting HLA Class 1 Antigen B27,B44,B47(X-Reactive). Reactive in Human. Host: Mouse. More details

$2075 / 0.1 mL +2 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
HLA Class 1 Antigen B27,B44,B47(X-Reactive), Monoclonal Antibody
Full Product Name
HLA Class 1 Antigen B27,B44,B47(X-Reactive)
Product Synonym Names
Anti -HLA Class 1 Antigen B27,B44,B47(X-Reactive)
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
4i115
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a lyophilized powder from PBS, 1% BSA. Reconstitute with100ul sterile ddH2O to make a 10X stock solution.
Purity/Purification
Ascites
Specificity
Reacts to HLA Class 1 Antigen-B27,B44,B47. Additional cross-reactivity occurs with related proteins. Specificity was determined at a 1:10 dilution by the microcytotoxicity test under standard NIH conditions. R-Value: 0.614
Preparation and Storage
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone No.
8.L.242
Expected Results
Cell death will occur in any test well where the HLA cell surface antigen is recognized by its matched anti-HLA antibody. Live lymphocytes indicate a negative reaction. Dead lymphocytes indicate a positive reaction.
Limitations
Cell isolation difficulties, contamination of the lymphocyte preparation with red blood cells, monocytes, platelets or granulocytes, cell concentrations outside acceptable levels, bacterial contamination and/or change in pH of antisera may cause erroneous results.

Product Categories/Family for anti-HLA Class 1 Antigen B27,B44,B47(X-Reactive) antibody

Antibodies; Abs to HLA
Applications Tested/Suitable for anti-HLA Class 1 Antigen B27,B44,B47(X-Reactive) antibody
This antibody has not been tested in methodologies other than Microcytotoxicity Test.
Potential applications include Flow Cytometry (FC), Cell Typing, Tissue Staining and Chimerism Studies.
Application Notes for anti-HLA Class 1 Antigen B27,B44,B47(X-Reactive) antibody
For a cytotoxic working dilution, add 0.9ml of 1% BSA in PBS to 100ul of10X working dilution. Store aliquots at -20°C.

Other Notes

Small volumes of anti-HLA Class 1 Antigen B27,B44,B47(X-Reactive) antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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