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Neuroglobin (NGB) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS603568

Neuroglobin, a 151 amino acid reside protein, mainly expressed in vertebrate brain and retina, is a recently identified member of the globin superfamily. Augmenting O2 supply, neuroglobin promotes survival of neurons upon hypoxic injury, potentially limiting… More details

$790 / 0.1 mg +1 FREE 8GB USB
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Neuroglobin (NGB), Monoclonal Antibody
Full Product Name
Neuroglobin
Product Gene Name
Product Synonym Names
Anti -Neuroglobin
Clonality
Monoclonal
Isotype
IgG1
Clone Number
5i332
Host
Mouse
Species Reactivity
Human
Form/Format
Sterile filtered and lyophilized from 1mg/ml 0.05M phosphate buffer, 0.1M sodium chloride, pH 7.2.
Purity/Purification
Affinity Purified
Affinity chromatography on a column with immobilized protein G
Specificity
The amino acid sequence of the recombinant human neuroglobin is 100% homologous with the amino acid sequence of the human neuroglobin.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
16,933 Da
Chromosome Location
Chromosome: 14; NC_000014.8 (77731834..77737655, complement). Location: 14q24.3
OMIM
605304
3D Structure
ModBase 3D Structure for Q9NPG2
Immunogen
Recombinant human neuroglobin
Reconstitution
Add 0.1ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of
the antibody. In this case clarify the solution by centrifugation.

Product Categories/Family for anti-NGB antibody

Applications Tested/Suitable for anti-NGB antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-NGB antibody
Suitable for use in ELISA, Western blotting.
Titer: Defined by indirect ELISA is >1:100,000 for antibody concentration
1mg/ml, 25ng of antigen are coated per well, and is then defined at a point of
maximal decrease of the titration curve.

Western Blot (WB) of anti-NGB antibody

WB analysis (12% gel; SDS-PAGE reducing conditions) using recombinant human neuroglobin as sample. MBS603568 was used at 1ug/ml. Secondary antibody used was HRP-conjugated anti-mouse IgG.|Lane 1: M.W. marker – 14, 21, 31, 45, 66, 97 kDa|Lane 2: reduced and heated sample, 0.1ug/lane |Lane 3: reduced and heated sample, 0.01ug/lane
Mouse Anti-NGB Monoclonal Antibody – Cat# MBS603568

Other Notes

Small volumes of anti-NGB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NGB purchase

MBS603568 is an antibody targeting NGB. Also known as Neuroglobin. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for NGB. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
neuroglobin
NCBI Official Synonym Full Names
neuroglobin
NCBI Official Symbol
NGB
NCBI Official Synonym Symbols
NGB
UniProt Protein Name
Neuroglobin
Protein Family
UniProt Gene Name
NGB
UniProt Entry Name
NGB_HUMAN
NCBI Protein Information
neuroglobin

NCBI Summary for NGB

This gene encodes an oxygen-binding protein that is distantly related to members of the globin gene family. It is highly conserved among other vertebrates. It is expressed in the central and peripheral nervous system where it may be involved in increasing oxygen availability and providing protection under hypoxic/ischemic conditions. [provided by RefSeq]
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UniProt Comments for NGB

NGB: Involved in oxygen transport in the brain. Hexacoordinate globin, displaying competitive binding of oxygen or the distal His residue to the iron atom. Not capable of penetrating cell membranes. The deoxygenated form exhibits nitrite reductase activity inhibiting cellular respiration via NO-binding to cytochrome c oxidase. Involved in neuroprotection during oxidative stress. May exert its anti-apoptotic activity by acting to reset the trigger level of mitochondrial cytochrome c release necessary to commit the cells to apoptosis. Belongs to the globin family.

Chromosomal Location of Human Ortholog: 14q24.3

Cellular Component: mitochondrion; perikaryon

Molecular Function: iron ion binding; heme binding; oxygen binding; oxygen transporter activity

Biological Process: apoptosis; oxygen transport
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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