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Remodeling and Spacing Factor, Large Subunit (RSF1) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS603727

Transcription of naked DNA in vitro requires the general transcription factors and RNA polymerase II. However, this minimal set of factors is not sufficient for transcription when the DNA template is packaged into chromatin. Here, a factor that facilitates… More details

$950 / 0.15 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Remodeling and Spacing Factor, Large Subunit (RSF1), Monoclonal Antibody
Full Product Name
Remodeling and Spacing Factor, Large Subunit (RSF)
Product Gene Name
anti-RSF1 antibody
Product Synonym Names
Anti -Remodeling and Spacing Factor, Large Subunit (RSF)
Clonality
Monoclonal
Isotype
IgG1
Clone Number
5i115
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Concentration
1 mg/mL (lot specific)
Specificity
Recognizes the large subunit (325kD) of human RSF complex. This complex is capable of facilitating ATP-dependent nucleosome remodeling and transcription initiation from chromatin templates.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Chromosome Location
Chromosome: 11; NC_000011.9 (77377274..77531880, complement). Location: 11q14.1
OMIM
608522
3D Structure
ModBase 3D Structure for Q96T23
Immunogen
Purified Remodeling and Spacing Factor, RSF.

Product Categories/Family for anti-RSF1 antibody

Antibodies; Abs to Transcription Factors
Applications Tested/Suitable for anti-RSF1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-RSF1 antibody
Suitable for use in ELISA, Western Blot, and Immunoprecipitation.
Dilution: ELISA: 1:2000
Western Blot: 1:2000

Other Notes

Small volumes of anti-RSF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RSF1 purchase

MBS603727 is an antibody targeting RSF1. Also known as Remodeling and Spacing Factor, Large Subunit (RSF). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for RSF1. It may not necessarily be applicable to this product.

NCBI GI #
251757329
NCBI GeneID
51773
UniProt Primary Accession #
Q96T23
UniProt Secondary Accession #
Q86X86; Q9H3L8; Q9NVZ8; Q9NYU0
UniProt Related Accession #
Q05DG0; Q96T23
NCBI Official Full Name
Remodeling and spacing factor 1
NCBI Official Synonym Full Names
remodeling and spacing factor 1
NCBI Official Symbol
RSF1
NCBI Official Synonym Symbols
XAP8; p325; HBXAP; RSF-1
UniProt Protein Name
Remodeling and spacing factor 1
UniProt Synonym Protein Names
HBV pX-associated protein 8; Hepatitis B virus X-associated protein; p325 subunit of RSF chromatin-remodeling complex
Protein Family
Remodeling and spacing factor
UniProt Gene Name
RSF1
UniProt Synonym Gene Names
HBXAP; XAP8
UniProt Entry Name
RSF1_HUMAN
NCBI Protein Information
remodeling and spacing factor 1; OTTHUMP00000197352; OTTHUMP00000233995; HBV pX associated protein-8; HBV pX-associated protein 8; hepatitis B virus x associated protein; hepatitis B virus x-associated protein; p325 subunit of RSF chromatin-remodeling complex

NCBI Summary for RSF1

HBXAP is involved in transcription repression, transcription coactivation when associated with hepatitis B virus X protein (HBX), and chromatin remodeling and spacing when associated with SNF2H (MIM 603375).[supplied by OMIM]

UniProt Comments for RSF1

HBXAP: Required for assembly of regular nucleosome arrays by the RSF chromatin-remodeling complex. Facilitates transcription of hepatitis B virus (HBV) genes by the pX transcription activator. In case of infection by HBV, together with pX, it represses TNF- alpha induced NF-kappa-B transcription activation. Represses transcription when artificially recruited to chromatin by fusion to a heterogeneous DNA binding domain. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 11q14.1

Cellular Component: nucleoplasm; RSF complex; nucleus

Molecular Function: protein binding; zinc ion binding; histone binding; ATPase activity

Biological Process: chromatin remodeling; nucleosome assembly; DNA replication-independent nucleosome assembly at centromere; positive regulation of viral transcription; positive regulation of transcription, DNA-dependent; transcription initiation; negative regulation of transcription, DNA-dependent; nucleosome positioning; negative regulation of DNA binding
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Research Articles on RSF1

1. a higher expression level of Rsf-1 is associated with advanced clinical stage and lymph node metastasis in ovarian clear-cell carcinoma.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RSF1 antibody

 Reference Product  PubMed Publications
 SMARCA5 antibody  >3 publications with RSF1 and SMARCA5

Pathways associated with anti-RSF1 antibody

 Products by Pathway  Pathway Diagram
 Chromosome Maintenance Pathway antibodies  Chromosome Maintenance Pathway Diagram
 Deposition Of New CENPA-containing Nucleosomes At The Centromere Pathway antibodies  Deposition Of New CENPA-containing Nucleosomes At The Centromere Pathway Diagram
 Nucleosome Assembly Pathway antibodies  Nucleosome Assembly Pathway Diagram

Diseases associated with anti-RSF1 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >14 publications with RSF1 and Neoplasms
 Carcinoma Antibodies  >11 publications with RSF1 and Carcinoma
 Gonadal Disorders Antibodies  >11 publications with RSF1 and Gonadal Disorders
 Adenocarcinoma Antibodies  >5 publications with RSF1 and Adenocarcinoma
 Breast Neoplasms Antibodies  >3 publications with RSF1 and Breast Neoplasms
 Liver Neoplasms Antibodies  >2 publications with RSF1 and Liver Neoplasms
 Esophageal Neoplasms Antibodies  >1 publications with RSF1 and Esophageal Neoplasms

Organs/Tissues associated with anti-RSF1 antibody

 Organ/Tissue Name  Pubmed Publications
 Lymph Antibodies  >2 publications with RSF1 and Lymph
 Lymph Node Antibodies  >2 publications with RSF1 and Lymph Node
 Liver Antibodies  >2 publications with RSF1 and Liver
 Blood Antibodies  >2 publications with RSF1 and Blood
 Eye Antibodies  >1 publications with RSF1 and Eye
 Embryonic Tissue Antibodies  >1 publications with RSF1 and Embryonic Tissue
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