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Ribonuclease Inhibitor 1 (RNH1) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS608354

Ribonuclease inhibitor 1(RNH1) the placental ribonuclease inhibitor, is an acid protein with a molecular weight of 50kD. RNH1 is a member of a family of proteinaceous cytoplasmic RNase inhibitors that are expressed in many tissues and bind to both… More details

$655 / 0.1 mL +1 FREE 8GB USB
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Ribonuclease inhibitor 1 (RNH1), Monoclonal Antibody
Full Product Name
Ribonuclease inhibitor 1 (RNH1)
Product Gene Name
Product Synonym Names
Anti -Ribonuclease inhibitor 1 (RNH1)
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
10C309
Host
Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 10% glycerol.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Concentration
1mg/ml (lot specific)
Specificity
Recognizes human Ribonuclease inhibitor 1
Preparation and Storage
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Chromosome Location
Chromosome: 11; NC_000011.9 (494512..507273, complement). Location: 11p15.5
OMIM
173320
3D Structure
ModBase 3D Structure for P13489
Immunogen
Recombinant protein corresponding to aa7-461 from human Ribonuclease inhibitor 1 expressed in E. coli.
Hybridoma
FO myeloma cells with spleen cells form Balb/c mice.
Applications Tested/Suitable for anti-RNH1 antibody
Immunofluoresence/Immunocytochemistry (IF/ICC), ELISA and Western Blot (WB).
Application Notes for anti-RNH1 antibody
WB: 1:500
IF/ICC: 1:100
Optimal dilutions to be determined by the researcher. Other applications not tested.

Western Blot of anti-RNH1 antibody

Western Blot analysis of cell lysates (40ug) were resolved by SDS-PAGE, trasferred to PVDF membrane using MBS608354 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP
Lane 1: Jurkat cell lysate
Lane 2: HepG2 cell lysate
Mouse Anti-RNH1 Monoclonal Antibody – Cat# MBS608354

Immunofluorescence (IFC) of anti-RNH1 antibody

Immunofluorescence (IFC) analysis of PC3 cells using MBS608354 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Mouse Anti-RNH1 Monoclonal Antibody, Immunofluorescence (IFC) – Cat# MBS608354

Other Notes

Small volumes of anti-RNH1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RNH1 purchase

MBS608354 is an antibody targeting RNH1. Also known as Ribonuclease inhibitor 1 (RNH1). Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for RNH1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
ribonuclease inhibitor
NCBI Official Synonym Full Names
ribonuclease/angiogenin inhibitor 1
NCBI Official Symbol
RNH1
NCBI Official Synonym Symbols
RAI; RNH; MGC4569; MGC18200; MGC54054
UniProt Protein Name
Ribonuclease inhibitor
UniProt Synonym Protein Names
Placental ribonuclease inhibitor; Placental RNase inhibitor; Ribonuclease/angiogenin inhibitor 1
Protein Family
UniProt Gene Name
RNH1
UniProt Synonym Gene Names
PRI; RNH
UniProt Entry Name
RINI_HUMAN
NCBI Protein Information
ribonuclease inhibitor; OTTHUMP00000147628; OTTHUMP00000229864; OTTHUMP00000229865; OTTHUMP00000229866; OTTHUMP00000229870; OTTHUMP00000229871; OTTHUMP00000229872; placental RNase inhibitor; Placental ribonuclease inhibitor

NCBI Summary for RNH1

Placental ribonuclease inhibitor (PRI) is a member of a family of proteinaceous cytoplasmic RNase inhibitors that occur in many tissues and bind to both intracellular and extracellular RNases (summarized by Lee et al., 1988 [PubMed 3219362]). In addition to control of intracellular RNases, the inhibitor may have a role in the regulation of angiogenin (MIM 105850). Ribonuclease inhibitor, of 50,000 Da, binds to ribonucleases and holds them in a latent form. Since neutral and alkaline ribonucleases probably play a critical role in the turnover of RNA in eukaryotic cells, RNH may be essential for control of mRNA turnover; the interaction of eukaryotic cells with ribonuclease may be reversible in vivo.[supplied by OMIM]
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UniProt Comments for RNH1

RNH1: Ribonuclease inhibitor which inhibits RNASE1, RNASE2 and ANG. May play a role in redox homeostasis.

Protein type: Inhibitor

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: angiogenin-PRI complex; cytoplasm

Molecular Function: protein binding; ribonuclease inhibitor activity

Biological Process: negative regulation of catalytic activity; mRNA catabolic process; regulation of angiogenesis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RNH1 antibody

 Reference Product  PubMed Publications
 ANG antibody  >2 publications with RNH1 and ANG
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