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Semaphorin 4d (SEMA4D) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS608547

Semaphorin-4D is an 863-aa polypeptide containing a secretory signal sequence, a single transmembrane domain, an immunoglobulin (Ig)-like domain, and a conserved 500-amino acid sema domain. It was first identified as a cell surface protein of resting T cells… More details

$750 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Semaphorin 4D (SEMA4D), Monoclonal Antibody
Full Product Name
Semaphorin 4D (BB18, A8, GR3, CD100 antigen, SEMA4D, CD100, SEMAJ)
Product Gene Name
anti-SEMA4D antibody
Product Synonym Names
Anti -Semaphorin 4D (BB18, A8, GR3, CD100 antigen, SEMA4D, CD100, SEMAJ)
Clonality
Monoclonal
Isotype
IgG
Clone Number
9G440
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with 100ul sterile ddH2O.
Purity/Purification
Affinity Purified
Purified by affinity chromatography.
Specificity
Recognizes human Semaphorin-4D.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile 40-50% glycerol, ddH2O. Reconstituted product is stable for 12 months at -20 degree C. Aliquot and store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
96,150 Da
Chromosome Location
Chromosome: 9; NC_000009.11 (91975706..92094611, complement). Location: 9q22.2
OMIM
601866
3D Structure
ModBase 3D Structure for Q92854
Immunogen
Partial recombinant human Semaphorin-4D (115-224) with a GST tag.

Product Categories/Family for anti-SEMA4D antibody

Antibodies; Abs to Plexin; Semaphorin
Applications Tested/Suitable for anti-SEMA4D antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-SEMA4D antibody
Suitable for use in ELISA and Western Blot.

Western Blot (WB) of anti-SEMA4D antibody

Western blot detection of human Semaphorin-4D expression in human promyelocytic leukemia whole cell lysate.
Mouse Anti-SEMA4D Monoclonal Antibody – Cat# MBS608547

ELISA (EIA) of anti-SEMA4D antibody

Sandwich ELISA using the anti-Semaphorin-4D monoclonal antibody as a capture antibody. The detection limit for GST tagged recombinant Semaphorin-4D is 0.3 ng/ml.
Mouse Anti-SEMA4D Monoclonal Antibody, ELISA (EIA) – Cat# MBS608547

Other Notes

Small volumes of anti-SEMA4D antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SEMA4D purchase

MBS608547 is an antibody targeting SEMA4D. Also known as Semaphorin 4D (BB18, A8, GR3, CD100 antigen, SEMA4D, CD100, SEMAJ). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for SEMA4D. It may not necessarily be applicable to this product.

NCBI GI #
214010218
NCBI GeneID
10507
NCBI Accession #
NP_006369.3
UniProt Primary Accession #
Q92854
UniProt Secondary Accession #
Q7Z5S4; Q8N8B0; B2RPM6
UniProt Related Accession #
Q4LE41; Q92854
GenBank Nucleotide #
NM_006378.3
NCBI Official Full Name
semaphorin-4D isoform 1
NCBI Official Synonym Full Names
sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
NCBI Official Symbol
SEMA4D
NCBI Official Synonym Symbols
CD100; SEMAJ; coll-4; C9orf164; FLJ33485; FLJ34282; FLJ39737; FLJ46484; M-sema-G; MGC169138; MGC169141
UniProt Protein Name
Semaphorin-4D
UniProt Synonym Protein Names
A8; BB18; GR3
Protein Family
Semaphorin
UniProt Gene Name
SEMA4D
UniProt Synonym Gene Names
C9orf164; CD100; SEMAJ
UniProt Entry Name
SEM4D_HUMAN
NCBI Protein Information
semaphorin-4D; A8; GR3; BB18; OTTHUMP00000208931; OTTHUMP00000208950; OTTHUMP00000208951; OTTHUMP00000208952; OTTHUMP00000208957; OTTHUMP00000240168; OTTHUMP00000240188; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, 4D

UniProt Comments for SEMA4D

SEMA4D: a single-pass type I membrane protein that my play a functional role in the immune system, as well as in the nervous system. Induces B-cells to aggregate and improves their viability in vitro.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 9q22.2

Cellular Component: extracellular space; integral to plasma membrane; plasma membrane

Molecular Function: protein binding; transmembrane receptor activity; receptor activity; semaphorin receptor binding; receptor binding

Biological Process: axon guidance; negative regulation of peptidyl-tyrosine phosphorylation; regulation of dendrite morphogenesis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of cell adhesion; positive regulation of phosphoinositide 3-kinase cascade; regulation of cell shape; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of osteoblast differentiation; immune response; positive regulation of protein amino acid phosphorylation; cell adhesion; positive regulation of collateral sprouting; regulation of cell projection organization and biogenesis; positive regulation of cell migration; negative regulation of apoptosis
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Research Articles on SEMA4D

1. crystal structures of cognate complexes of the semaphorin-binding regions of plexins B1 and A2 with semaphorin ectodomains (human PLXNB1(1-2)-SEMA4D(ecto) and murine PlxnA2(1-4)-Sema6A(ecto)), plus unliganded structures of PlxnA2(1-4) and Sema6A(ecto)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SEMA4D antibody

 Reference Product  PubMed Publications
 PLXNB1 antibody  >46 publications with SEMA4D and PLXNB1
 CD72 antibody  >29 publications with SEMA4D and CD72
 PTPRC antibody  >4 publications with SEMA4D and PTPRC

Pathways associated with anti-SEMA4D antibody

 Products by Pathway  Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Sema4D In Semaphorin Signaling Pathway antibodies  Sema4D In Semaphorin Signaling Pathway Diagram
 Sema4D Induced Cell Migration And Growth-cone Collapse Pathway antibodies  Sema4D Induced Cell Migration And Growth-cone Collapse Pathway Diagram
 Sema4D Mediated Inhibition Of Cell Attachment And Migration Pathway antibodies  Sema4D Mediated Inhibition Of Cell Attachment And Migration Pathway Diagram
 Semaphorin Interactions Pathway antibodies  Semaphorin Interactions Pathway Diagram

Diseases associated with anti-SEMA4D antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >19 publications with SEMA4D and Neoplasms
 Inflammation Antibodies  >7 publications with SEMA4D and Inflammation
 Kidney Diseases Antibodies  >4 publications with SEMA4D and Kidney Diseases
 Skin Diseases Antibodies  >3 publications with SEMA4D and Skin Diseases
 Lung Neoplasms Antibodies  >1 publications with SEMA4D and Lung Neoplasms
 Reperfusion Injury Antibodies  >1 publications with SEMA4D and Reperfusion Injury
 Asthma Antibodies  >1 publications with SEMA4D and Asthma
 Neurodegenerative Diseases Antibodies  >1 publications with SEMA4D and Neurodegenerative Diseases
 Melanoma, Experimental Antibodies  >1 publications with SEMA4D and Melanoma, Experimental
 Iron Metabolism Disorders Antibodies  >1 publications with SEMA4D and Iron Metabolism Disorders

Organs/Tissues associated with anti-SEMA4D antibody

 Organ/Tissue Name  Pubmed Publications
 Nerve Antibodies  >65 publications with SEMA4D and Nerve
 Blood Antibodies  >52 publications with SEMA4D and Blood
 Brain Antibodies  >18 publications with SEMA4D and Brain
 Embryonic Tissue Antibodies  >13 publications with SEMA4D and Embryonic Tissue
 Kidney Antibodies  >7 publications with SEMA4D and Kidney
 Spinal Cord Antibodies  >4 publications with SEMA4D and Spinal Cord
 Lung Antibodies  >3 publications with SEMA4D and Lung
 Bone Marrow Antibodies  >3 publications with SEMA4D and Bone Marrow
 Pharynx Antibodies  >3 publications with SEMA4D and Pharynx
 Ganglia Antibodies  >3 publications with SEMA4D and Ganglia
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