Rabbit Anti-PPH22 Polyclonal Antibody – Cat# MBS611697

Protein Serine/Threonine Phosphatase 2a/A (PPH22) Antibody – Rabbit Polyclonal

Name: PPH22 Antibody; Polyclonal; Rabbit; MyBioSource
Brand: MyBioSource
SKU: MBS611697
Price: 940 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS611697

MBS611697 is an antibody targeting PPH22. Also known as Protein Serine/Threonine phosphatase 2A/A. Reactive in Bovine, Human, Mouse, Rat. Host: Rabbit. More details

$940 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Protein Serine/Threonine phosphatase 2A/A (PPH22), Polyclonal Antibody

Full Product Name

Protein Serine/Threonine phosphatase 2A/A

Product Gene Name

anti-PPH22 antibody

Product Synonym Names

Anti -Protein Serine/Threonine phosphatase 2A/A

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Bovine, Human, Mouse, Rat

Form/Format

Supplied as a liquid in PBS, 0.08% sodium azide.

Purity/Purification

Purified
Purified

Specificity

Recognizes human PP 2A/A at 65kD. Species Crossreactivity: mouse, rat and bovine. Does not crossreact with other protein phosphatase isoforms.

Preparation and Storage

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight

43,047 Da

3D Structure

ModBase 3D Structure for P23595

Immunogen

Synthetic peptide corresponding to aa7-19, NH2-Asp-Asp-Ser-Leu-Tyr-Pro- Ile-Ala-Val-Leu-Ile-Asp-Glu-COOH, from the N-terminal of PP 2A/A regulatory subunit

Product Categories/Family for anti-PPH22 antibody

Antibodies; Abs to Enzymes; Phosphatase

Applications Tested/Suitable for anti-PPH22 antibody

Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Application Notes for anti-PPH22 antibody

Suitable for use in Western Blot, Immunocytochemistry, and Immunoprecipitation.
Dilution: Western Blot: 5-10ug/ml
Immunocytochemistry: 10-15ug/ml
Immunoprecipitation: Can be used in conjunction with anti-PP2A/C to immunoprecipitate heterotrimeric PP2A
Optimum dilutions to be determined by researcher.

Other Notes

Small volumes of anti-PPH22 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PPH22. It may not necessarily be applicable to this product.

NCBI GI #

4210

NCBI Accession #

CAA39703.1

UniProt Primary Accession #

P23595

UniProt Secondary Accession #

D6VRG5

NCBI Official Full Name

protein serine /threonine phosphatase 2A

UniProt Protein Name

Serine/threonine-protein phosphatase PP2A-2 catalytic subunit

UniProt Gene Name

PPH22

UniProt Synonym Gene Names

SIS4

UniProt Entry Name

PP2A2_YEAST

UniProt Comments for PPH22

Function: Exact function not known, phosphatase 2A performs an essential cellular function.

Catalytic activity: A phosphoprotein + H2O = a protein + phosphate.

Cofactor: Binds 1 iron ion per subunit

By similarity.Binds 1 manganese ion per subunit

By similarity.

Subunit structure: Inactivated in a complex with phosphatase methylesterase PPE1 (PP2Ai). Interacts with phosphatase 2A activator RRD2, which can reactivate PP2Ai by dissociating the catalytic subunit from the complex. Interacts with TAP42. Ref.9 Ref.11

Post-translational modification: Reversibly methyl esterified on Leu-377 by leucine carboxyl methyltransferase 1 (PPM1) and protein phosphatase methylesterase 1 (PPE1). Carboxyl methylation influences the affinity of the catalytic subunit for the different regulatory subunits, thereby modulating the PP2A holoenzyme's substrate specificity, enzyme activity and cellular localization.

Miscellaneous: Present with 4110 molecules/cell in log phase SD medium.

Sequence similarities: Belongs to the PPP phosphatase family. PP-2A subfamily.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PPH22 antibody

Reference Product	PubMed Publications
PPH21 antibody	>18 publications with PPH22 and PPH21
CDC55 antibody	>6 publications with PPH22 and CDC55
TAP42 antibody	>5 publications with PPH22 and TAP42
TPD3 antibody	>2 publications with PPH22 and TPD3
RTS1 antibody	>2 publications with PPH22 and RTS1
AMN1 antibody	>1 publications with PPH22 and AMN1
TEM1 antibody	>1 publications with PPH22 and TEM1