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Interleukin 2 Receptor Alpha (IL-2sRa) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6121813

MBS6121813 is an antibody targeting IL-2sRa. Also known as Interleukin 2 Receptor alpha, Soluble (IL-2sRa) (AP). Reactive in Human. Host: Mouse. More details

$940 / 0.1 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Interleukin 2 Receptor alpha (IL-2sRa), Monoclonal Antibody
Full Product Name
Interleukin 2 Receptor alpha, Soluble (IL-2sRa) (AP)
Product Gene Name
anti-IL-2sRa antibody
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
7F4
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Purity/Purification
Purified by Protein G Affinity Chromatography
Specificity
Recognizes human sIL-2 Receptor.
Preparation and Storage
May be stored at 4 degree C before opening. DO NOT FREEZE! Stable at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing alkaline phosphatase conjugates will result in a substantial loss of activity.
Immunogen
Purified recombinant, insect cell derived, human soluble IL-2 Receptor alpha
Conjugate
AP

Product Categories/Family for anti-IL-2sRa antibody

Antibodies; Receptors
Applications Tested/Suitable for anti-IL-2sRa antibody
ELISA (EIA), Neutralization, Western Blot (WB)
Application Notes for anti-IL-2sRa antibody
ELISA: To detect hsIL-2Ralpha by direct ELISA (using 100ul/well antibody solution) a concentration of at least 4ug/ml of this antibody is required. This monoclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.1ng/well of recombinant hsIL-2Ralpha.
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hsIL-2Ralpha (100ng/ml), in the presence of 0.5ng/ml of hIL-2, a concentration of 1.0-1.5ug/ml of this antibody is required.
WB: To detect hsIL-2Ralpha by Western Blot analysis this antibody can be used at a concentration of 0.20ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsIL-2Ra is 1.95-3.0ng/lane, under non-reducing conditions.
Applications are based on unconjugated antibody.

Other Notes

Small volumes of anti-IL-2sRa antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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