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MART-1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6124353

MBS6124353 is an antibody targeting MART-1. Also known as MART-1 (Antigen LB39-AA, Antigen SK29-AA, Melanoma antigen recognized by T-cells 1, MLAN-A, MLANA, Melan-A) (PE). Reactive in Human. Host: Mouse. More details

$1105 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MART-1, Monoclonal Antibody
Full Product Name
MART-1 (Antigen LB39-AA, Antigen SK29-AA, Melanoma antigen recognized by T-cells 1, MLAN-A, MLANA, Melan-A) (PE)
Product Gene Name
Product Synonym Names
Antigen LB39-AA; Antigen SK29-AA; Melanoma antigen recognized by T-cells 1; MLAN-A; MLANA; Melan-A
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
SPM342
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with R-Phycoerythrin (PE).
Purity/Purification
Purified by Protein A/G Affinity Chromatography.
Specificity
Recognizes human MART-1. Does not react with mouse and rat.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
13,157 Da
Chromosome Location
Chromosome: 9; NC_000009.11 (5890909..5909822). Location: 9p24.1
OMIM
605513
3D Structure
ModBase 3D Structure for Q16655
Immunogen
Recombinant hMART-1 protein. Cellular Localization: Cytoplasmic
Conjugate
PE
Positive Control
SK-MEL-13 and SK-MEL-19 Melanoma cell lines; Melanomas
Special Handling
Light sensitive

Product Categories/Family for anti-MART-1 antibody

Applications Tested/Suitable for anti-MART-1 antibody
Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-MART-1 antibody
FC/FACS: 0.5-1ug/million cells
IP: 1-2ug/500ug protein lysate
IF: 1-2ug/ml
IHC: 0.5-1ug/ml for 30 minutes at RT
Applications are based on unconjugated antibody.

Immunohistochemistry (IHC) of anti-MART-1 antibody

Formalin-paraffin human melanoma stained with MART-1 Ab (SPM342). Note cytoplasmic staining of cells.
Mouse Anti-MART-1 Monoclonal Antibody – Cat# MBS6124353

Immunohistochemistry (IHC) of anti-MART-1 antibody

Formalin-paraffin human melanoma stained with MART-1 Ab (SPM342). Note cytoplasmic staining of cells.
Mouse Anti-MART-1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6124353

Product References and Citations for anti-MART-1 antibody

1. Kawakami Y, et. al. Journal of Immunological Methods, 1997, 202(1):13-25. 2. Marincola FM, et. al. Journal of Immunotherapy with Emphasis on Tumor Immunology, 1996, 19(3):192-205.

Other Notes

Small volumes of anti-MART-1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MART-1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
melanoma antigen recognized by T-cells 1
NCBI Official Synonym Full Names
melan-A
NCBI Official Symbol
MLANA
NCBI Official Synonym Symbols
MART1; MART-1
UniProt Protein Name
Melanoma antigen recognized by T-cells 1
UniProt Synonym Protein Names
Antigen LB39-AA; Antigen SK29-AA; Protein Melan-A
UniProt Gene Name
MLANA
UniProt Synonym Gene Names
MART1; MART-1
UniProt Entry Name
MAR1_HUMAN
NCBI Protein Information
melanoma antigen recognized by T-cells 1; antigen LB39-AA; antigen SK29-AA; protein Melan-A

UniProt Comments for MART-1

MLANA: Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 9p24.1

Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; integral to plasma membrane; melanosome; trans-Golgi network

Molecular Function: protein binding
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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