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RAD54B Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6133296

MBS6133296 is an antibody targeting RAD54B. Also known as RAD54B (DNA Replication, Repair and Recombination Factor) (AP). Reactive in Human. Host: Mouse. More details

$995 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
RAD54B, Monoclonal Antibody
Full Product Name
RAD54B (DNA Replication, Repair and Recombination Factor) (AP)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
4A7
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human RAD54B.
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
13,330 Da
Immunogen
Partial recombinant corresponding to aa801-910 from RAD54B (AAH01965) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
HIQFSVEELKNLFTLHESSDCVTHDLLDCECTGEEVHTGDSLEKFIVSRDCQLGPHHQKSNSLKPLSMSQLKQWKHFSGDHLNLTDPFLERITENVSFIFQNITTQATGT
Conjugate
AP

Product Categories/Family for anti-RAD54B antibody

Applications Tested/Suitable for anti-RAD54B antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-RAD54B antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-RAD54B antibody

Western Blot detection against Immunogen (38.1kD).
Mouse Anti-RAD54B Monoclonal Antibody – Cat# MBS6133296

Western Blot (WB) of anti-RAD54B antibody

RAD54B monoclonal antibody. Western Blot analysis of RAD54B expression in HeLa NE.
Mouse Anti-RAD54B Monoclonal Antibody, Western Blot (WB) – Cat# MBS6133296

Immunofluorescence (IF) of anti-RAD54B antibody

Immunofluorescence of monoclonal antibody to RAD54B on HeLa cell. [antibody concentration 10ug/ml]
Mouse Anti-RAD54B Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6133296

Testing Data of anti-RAD54B antibody

Detection limit for recombinant GST tagged RAD54B is ~0.03ng/ml as a capture antibody.
Mouse Anti-RAD54B Monoclonal Antibody, Testing Data – Cat# MBS6133296

Other Notes

Small volumes of anti-RAD54B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for RAD54B. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Homo sapiens RAD54 homolog B (S. cerevisiae), mRNA
NCBI Official Synonym Full Names
RAD54 homolog B (S. cerevisiae)
NCBI Official Symbol
RAD54B
NCBI Official Synonym Symbols
RDH54
NCBI Protein Information
DNA repair and recombination protein RAD54B

NCBI Summary for RAD54B

The protein encoded by this gene belongs to the DEAD-like helicase superfamily. It shares similarity with Saccharomyces cerevisiae RAD54 and RDH54, both of which are involved in homologous recombination and repair of DNA. This protein binds to double-stranded DNA, and displays ATPase activity in the presence of DNA. This gene is highly expressed in testis and spleen, which suggests active roles in meiotic and mitotic recombination. Homozygous mutations of this gene were observed in primary lymphoma and colon cancer. [provided by RefSeq, Jul 2008]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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