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Phosphodiesterase 5a (PDE5A) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6138226

Phosphodiesterase 5A, a cGMP-specific phosphodiesterase, has been implicated in primary pulmonary hypertension, cardiac failure and erectile dysfunction. PDE5A also may function in the regulation of coronary blood flow. PDE5A is the predominant enzyme… More details

$1155 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Phosphodiesterase 5A (PDE5A), Monoclonal Antibody
Full Product Name
Phosphodiesterase 5A (PDE5A, cGMP-Specific 3',5'-Cyclic Phosphodiesterase, cGMP-Binding cGMP-Specific Phosphodiesterase, CGB-PDE, PDE5) APC
Product Gene Name
anti-PDE5A antibody
Clonality
Monoclonal
Isotype
IgG3,k
Clone Number
9H5
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human Phosphodiesterase 5A.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
94,805 Da
OMIM
AF043731 mRNA
3D Structure
ModBase 3D Structure for O76074
Immunogen
Partial recombinant protein corresponding to aa29-138 from human Phosphodiesterase 5A with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
SVEAWLDDHWDFTFSYFVRKATREMVNAWFAERVHTIPVCKEGIRGHTESCSCPLQQSPRADNSVPGTPTRKISASEFDRPLRPIVVKDSEGTVSFLSDSEKKEQMPLTP*
Conjugate
APC

Product Categories/Family for anti-PDE5A antibody

Antibodies; Enzymes; Phosphodiesterase
Applications Tested/Suitable for anti-PDE5A antibody
FLISA, Immunofluorescence (IF), Western Blot (WB)
Application Notes for anti-PDE5A antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-PDE5A antibody

Western Blot detection against immunogen (38.21kD).
Mouse Anti-PDE5A Monoclonal Antibody – Cat# MBS6138226

Testing Data of anti-PDE5A antibody

Detection limit for 131278 is 1ng/ml as a capture antibody.
Mouse Anti-PDE5A Monoclonal Antibody, Testing Data – Cat# MBS6138226

Other Notes

Small volumes of anti-PDE5A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PDE5A purchase

MBS6138226 is an antibody targeting PDE5A. Also known as Phosphodiesterase 5A (PDE5A, cGMP-Specific 3',5'-Cyclic Phosphodiesterase, cGMP-Binding cGMP-Specific Phosphodiesterase, CGB-PDE, PDE5) APC. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for PDE5A. It may not necessarily be applicable to this product.

NCBI GI #
61744435
NCBI GeneID
8654
NCBI Accession #
NP_001074
UniProt Primary Accession #
O76074
UniProt Secondary Accession #
O75026; O75887; Q86UI0; Q86V66; Q9Y6Z6; A0AV69; A8K2C4
UniProt Related Accession #
O76074
GenBank Nucleotide #
NM_001083
NCBI Official Full Name
cGMP-specific 3',5'-cyclic phosphodiesterase isoform 1
NCBI Official Synonym Full Names
phosphodiesterase 5A
NCBI Official Symbol
PDE5A
NCBI Official Synonym Symbols
CN5A; PDE5; CGB-PDE
UniProt Protein Name
cGMP-specific 3',5'-cyclic phosphodiesterase
UniProt Synonym Protein Names
cGMP-binding cGMP-specific phosphodiesterase; CGB-PDE
UniProt Gene Name
PDE5A
UniProt Synonym Gene Names
PDE5; CGB-PDE
NCBI Protein Information
cGMP-specific 3',5'-cyclic phosphodiesterase

NCBI Summary for PDE5A

This gene encodes a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Alternative splicing of this gene results in three transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]
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UniProt Comments for PDE5A

PDE5A: a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Phosphorylation is regulated by binding of cGMP to the two allosteric sites. Sildenafil (Viagra) is a highly selective and potent inhibitor of PDE5A. Alternative splicing results in four transcript variants encoding distinct isoforms.

Protein type: EC 3.1.4.35; Nucleotide Metabolism - purine; Phosphodiesterase

Chromosomal Location of Human Ortholog: 4q26

Cellular Component: cytosol

Molecular Function: 3',5'-cyclic-GMP phosphodiesterase activity; cGMP binding

Biological Process: cGMP catabolic process
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Research Articles on PDE5A

1. Results show that Ser102 and Ser104 may influence the conformational flexibility of PDE5A, which may in turn influence phosphorylation status, allosteric regulation by cGMP or other as yet unknown regulatory mechanisms for PDE5A.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PDE5A antibody

 Reference Product  PubMed Publications
 CASP3 antibody  >6 publications with PDE5A and CASP3

Pathways associated with anti-PDE5A antibody

 Products by Pathway  Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Nitric Oxide Stimulates Guanylate Cyclase Pathway antibodies  Nitric Oxide Stimulates Guanylate Cyclase Pathway Diagram
 Platelet Homeostasis Pathway antibodies  Platelet Homeostasis Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 CGMP Effects Pathway antibodies  CGMP Effects Pathway Diagram
 CGMP-PKG Signaling Pathway antibodies  CGMP-PKG Signaling Pathway Diagram
 CGMP-PKG Signaling Pathway antibodies  CGMP-PKG Signaling Pathway Diagram

Diseases associated with anti-PDE5A antibody

 Disease Name  Pubmed Publications
 Erectile Dysfunction Antibodies  >271 publications with PDE5A and Erectile Dysfunction
 Sexual Dysfunction, Physiological Antibodies  >223 publications with PDE5A and Sexual Dysfunction, Physiological
 Cardiovascular Diseases Antibodies  >134 publications with PDE5A and Cardiovascular Diseases
 Hypertension Antibodies  >115 publications with PDE5A and Hypertension
 Heart Diseases Antibodies  >80 publications with PDE5A and Heart Diseases
 Hypertension, Pulmonary Antibodies  >79 publications with PDE5A and Hypertension, Pulmonary
 Respiratory Tract Diseases Antibodies  >68 publications with PDE5A and Respiratory Tract Diseases
 Myocardial Ischemia Antibodies  >34 publications with PDE5A and Myocardial Ischemia
 Headache Antibodies  >34 publications with PDE5A and Headache
 Coronary Disease Antibodies  >28 publications with PDE5A and Coronary Disease

Organs/Tissues associated with anti-PDE5A antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >200 publications with PDE5A and Muscle
 Vascular Antibodies  >196 publications with PDE5A and Vascular
 Heart Antibodies  >129 publications with PDE5A and Heart
 Lung Antibodies  >48 publications with PDE5A and Lung
 Brain Antibodies  >47 publications with PDE5A and Brain
 Nerve Antibodies  >33 publications with PDE5A and Nerve
 Kidney Antibodies  >24 publications with PDE5A and Kidney
 Prostate Antibodies  >20 publications with PDE5A and Prostate
 Testis Antibodies  >13 publications with PDE5A and Testis
 Eye Antibodies  >10 publications with PDE5A and Eye
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