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DHX8 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6141538

MBS6141538 is an antibody targeting DHX8. Also known as DHX8 (DEAH (Asp-Glu-Ala-His) Box Polypeptide 8, DEAH Box Protein 8, DHX-8, DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 8, DDX8, DDX-8, HRH1, Human RNA Helicase 1, PRP22, PRPF22) (Biotin). Reactive in… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
DHX8, Monoclonal Antibody
Full Product Name
DHX8 (DEAH (Asp-Glu-Ala-His) Box Polypeptide 8, DEAH Box Protein 8, DHX-8, DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 8, DDX8, DDX-8, HRH1, Human RNA Helicase 1, PRP22, PRPF22) (Biotin)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
1E10
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human DHX8.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
139kDa
OMIM
600396
3D Structure
ModBase 3D Structure for Q14562
Immunogen
Partial recombinant corresponding to aa301-400 from human DHX8 (NP_004932) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
RREGRVANVADVVSKGQRVKVKVLSFTGTKTSLSMKDVDQETGEDLNPNRRRNLVGETNEETSMRNPDRPTHLSLVSAPEVEDDSLERKRLTRISDPEKW
Conjugate
Biotin

Product Categories/Family for anti-DHX8 antibody

Applications Tested/Suitable for anti-DHX8 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-DHX8 antibody
Sandwich ELISA: The detection limit is ~0.3ng/ml as a capture antibody.
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-DHX8 antibody

Western Blot detection against Immunogen (36.74kD).
Mouse Anti-DHX8 Monoclonal Antibody – Cat# MBS6141538

Western Blot (WB) of anti-DHX8 antibody

DHX8 monoclonal antibody Western Blot analysis of DHX8 expression in Hela NE.
Mouse Anti-DHX8 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6141538

Testing Data of anti-DHX8 antibody

Detection limit for recombinant GST tagged DHX8 is ~0.3ng/ml as a capture antibody.
Mouse Anti-DHX8 Monoclonal Antibody, Testing Data – Cat# MBS6141538

Other Notes

Small volumes of anti-DHX8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for DHX8. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
ATP-dependent RNA helicase DHX8 isoform 1
NCBI Official Synonym Full Names
DEAH-box helicase 8
NCBI Official Symbol
DHX8
NCBI Official Synonym Symbols
DDX8; Dhr2; HRH1; PRP22; PRPF22
UniProt Protein Name
ATP-dependent RNA helicase DHX8
UniProt Synonym Protein Names
DEAH box protein 8; RNA helicase HRH1
UniProt Gene Name
DHX8
UniProt Synonym Gene Names
DDX8
UniProt Entry Name
DHX8_HUMAN
NCBI Protein Information
ATP-dependent RNA helicase DHX8

NCBI Summary for DHX8

This gene is a member of the DEAH box polypeptide family. The encoded protein contains the DEAH (Asp-Glu-Ala-His) motif which is characteristic of all DEAH box proteins, and is thought to function as an ATP-dependent RNA helicase that regulates the release of spliced mRNAs from spliceosomes prior to their export from the nucleus. This protein may be required for the replication of human immunodeficiency virus type 1 (HIV-1). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]
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UniProt Comments for DHX8

DDX8: Facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome. Belongs to the DEAD box helicase family. DEAH subfamily. DDX8/PRP22 sub-subfamily.

Protein type: RNA-binding; RNA processing; RNA splicing; EC 3.6.4.13; Helicase; Spliceosome

Chromosomal Location of Human Ortholog: 17q21.31

Cellular Component: nucleoplasm; spliceosome; cytoplasm; nucleus

Molecular Function: identical protein binding; protein binding; ATP binding; ATP-dependent RNA helicase activity

Biological Process: RNA processing; nuclear mRNA splicing, via spliceosome; RNA splicing
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-DHX8 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1 publications with DHX8 and Blood
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