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MCM7 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6142863

MBS6142863 is an antibody targeting MCM7. Also known as MCM7 (DNA Replication Licensing Factor MCM7, CDC47 Homolog, P1.1-MCM3, CDC47, MCM2) (Biotin). Reactive in Human. Host: Mouse. More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MCM7, Monoclonal Antibody
Full Product Name
MCM7 (DNA Replication Licensing Factor MCM7, CDC47 Homolog, P1.1-MCM3, CDC47, MCM2) (Biotin)
Product Gene Name
anti-MCM7 antibody
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
6C2
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human MCM7.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
60,643 Da
Immunogen
Full length recombinant corresponding to aa1-389 from human MCM7 (AAH09398) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MALKDYALEKEKVKKFLQEFYQDDELGKKQFKYGNQLVRLAHREQVALYVDLDDVAEDDPELVDSICENARRYAKLFADAVQELLPQYKEREVVNKDVLDVYIEHRLMMEQRSRDPGMVRSPQNQYPAELMRRFELYFQGPSSSKPRVIREVRADSVGKLVTVRGIVTRVSEVKPKMVVATYTCDQCGAETYQPIQSPTFMPLIMCPSQECQTNRSGGRLYLQTRGSRFIKFQEMKMQEHSDQVPVGNIPRSITVLVEGENTRIAQPGDHVSVTGIFLPILRTGFRQVVQGLLSETYLEAHRIVKMNKSEDDESGAGELTREELRQIADVIFATVRELVSGGRSVRFSEAEQRCVSRGFTPAQFQAALDEYEELNVWQVNASRTRITFV
Conjugate
Biotin

Product Categories/Family for anti-MCM7 antibody

Antibodies; DNA Replication; Repair
Applications Tested/Suitable for anti-MCM7 antibody
ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-MCM7 antibody
IF: 10ug/ml
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-MCM7 antibody

Western Blot detection against Immunogen (68.53kD).
Mouse Anti-MCM7 Monoclonal Antibody – Cat# MBS6142863

Western Blot (WB) of anti-MCM7 antibody

MCM7 monoclonal antibody Western Blot analysis of MCM7 expression in Hela NE.
Mouse Anti-MCM7 Monoclonal Antibody, Western Blot (WB) – Cat# MBS6142863

Immunohistochemistry (IHC) of anti-MCM7 antibody

Immunoperoxidase of monoclonal antibody to MCM7 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma. [antibody concentration 3ug/ml]
Mouse Anti-MCM7 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6142863

Immunofluorescence (IF) of anti-MCM7 antibody

Immunofluorescence of monoclonal antibody to MCM7 on HeLa cell. [antibody concentration 10ug/ml]
Mouse Anti-MCM7 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6142863

Testing Data of anti-MCM7 antibody

Detection limit for recombinant GST tagged MCM7 is ~0.1ng/ml as a capture antibody.
Mouse Anti-MCM7 Monoclonal Antibody, Testing Data – Cat# MBS6142863

Testing Data of anti-MCM7 antibody

Proximity Ligation Analysis of protein-protein interactions between CDK7 and MCM7 HeLa cells were stained with anti-CDK7 rabbit purified polyclonal 1:1200 and anti-MCM7 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Mouse Anti-MCM7 Monoclonal Antibody, Testing Data – Cat# MBS6142863

Testing Data of anti-MCM7 antibody

Proximity Ligation Analysis of protein-protein interactions between CDC6 and MCM7. Huh7 cells were stained with anti-CDC6 rabbit purified polyclonal 1:600 and anti-MCM7 mouse monoclonal antibody 1:100. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Mouse Anti-MCM7 Monoclonal Antibody, Testing Data – Cat# MBS6142863

Product References and Citations for anti-MCM7 antibody

1. Etiological role of human papillomavirus infection for inverted papilloma of the bladder. Shigehara K, Sasagawa T, Doorbar J, Kawaguchi S, Kobori Y, Nakashima T, Shimamura M, Maeda Y, Miyagi T, Kitagawa Y, Kadono Y, Konaka H, Mizokami A, Koh E, Namiki M.J Med Virol. 2011 Feb;83(2):277-85. 2. Etiologic role of human papillomavirus infection in bladder carcinoma. Shigehara K, Sasagawa T, Kawaguchi S, Nakashima T, Shimamura M, Maeda Y, Konaka H, Mizokami A, Koh E, Namiki M.Cancer. 2010 Nov 29. 3. Minichromosome maintenance proteins 2, 3 and 7 in medulloblastoma: overexpression and involvement in regulation of cell migration and invasion. Lau KM, Chan QK, Pang JC, Li KK, Yeung WW, Chung NY, Lui PC, Tam YS, Li HM, Zhou L, Wang Y, Mao Y, Ng HK.Oncogene. 2010 Jul 26. 4. Validation of different replication markers for the detection of beta-cell proliferation in human pancreatic tissue. Kohler CU, Kreuter A, Rozynkowski MC, Rahmel T, Uhl W, Tannapfel A, Schmidt WE, Meier JJ.Regul Pept. 2010 Jan 14.

Other Notes

Small volumes of anti-MCM7 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MCM7. It may not necessarily be applicable to this product.

NCBI GI #
33988389
NCBI GeneID
4176
NCBI Accession #
BC009398
NCBI Related Accession #
AAH09398
UniProt Secondary Accession #
Q15076; Q96D34; Q96GL1; A4D2A1; A4D2A2; E9PGN9
UniProt Related Accession #
P33993
NCBI Official Full Name
Homo sapiens minichromosome maintenance complex component 7, mRNA
NCBI Official Synonym Full Names
minichromosome maintenance complex component 7
NCBI Official Symbol
MCM7
NCBI Official Synonym Symbols
MCM2; CDC47; P85MCM; P1CDC47; PNAS146; PPP1R104; P1.1-MCM3
Protein Family
DNA replication licensing factor
NCBI Protein Information
DNA replication licensing factor MCM7

NCBI Summary for MCM7

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
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Research Articles on MCM7

1. Purified MCM4/6/7 complex containing the G364R MCM4 exhibited similar levels of single-stranded DNA binding and ATPase activities to the complex containing wild-type MCM4

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-MCM7 antibody

 Products by Pathway  Pathway Diagram
 Activation Of ATR In Response To Replication Stress Pathway antibodies  Activation Of ATR In Response To Replication Stress Pathway Diagram
 Activation Of The Pre-replicative Complex Pathway antibodies  Activation Of The Pre-replicative Complex Pathway Diagram
 Assembly Of The Pre-replicative Complex Pathway antibodies  Assembly Of The Pre-replicative Complex Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Checkpoints Pathway antibodies  Cell Cycle Checkpoints Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram

Diseases associated with anti-MCM7 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >105 publications with MCM7 and Neoplasms
 Carcinoma Antibodies  >49 publications with MCM7 and Carcinoma
 Adenocarcinoma Antibodies  >26 publications with MCM7 and Adenocarcinoma
 Prostatic Neoplasms Antibodies  >17 publications with MCM7 and Prostatic Neoplasms
 Uterine Neoplasms Antibodies  >12 publications with MCM7 and Uterine Neoplasms
 Breast Neoplasms Antibodies  >11 publications with MCM7 and Breast Neoplasms
 Carcinoma, Squamous Cell Antibodies  >11 publications with MCM7 and Carcinoma, Squamous Cell
 Lung Neoplasms Antibodies  >10 publications with MCM7 and Lung Neoplasms
 Uterine Cervical Neoplasms Antibodies  >8 publications with MCM7 and Uterine Cervical Neoplasms
 Skin Neoplasms Antibodies  >7 publications with MCM7 and Skin Neoplasms

Organs/Tissues associated with anti-MCM7 antibody

 Organ/Tissue Name  Pubmed Publications
 Prostate Antibodies  >21 publications with MCM7 and Prostate
 Lung Antibodies  >15 publications with MCM7 and Lung
 Skin Antibodies  >13 publications with MCM7 and Skin
 Vascular Antibodies  >12 publications with MCM7 and Vascular
 Blood Antibodies  >11 publications with MCM7 and Blood
 Muscle Antibodies  >9 publications with MCM7 and Muscle
 Placenta Antibodies  >8 publications with MCM7 and Placenta
 Liver Antibodies  >8 publications with MCM7 and Liver
 Brain Antibodies  >8 publications with MCM7 and Brain
 Embryonic Tissue Antibodies  >7 publications with MCM7 and Embryonic Tissue
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