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SPINT2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6144560

MBS6144560 is an antibody targeting SPINT2. Also known as SPINT2 (Kunitz-type Protease Inhibitor 2, Hepatocyte Growth Factor Activator Inhibitor Type 2, HAI-2, HAI2, KOP, Placental Bikunin, PB, DIAR3) (Biotin). Reactive in Human. Host: Mouse. More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SPINT2, Monoclonal Antibody
Full Product Name
SPINT2 (Kunitz-type Protease Inhibitor 2, Hepatocyte Growth Factor Activator Inhibitor Type 2, HAI-2, HAI2, KOP, Placental Bikunin, PB, DIAR3) (Biotin)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
2D1
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human SPINT2.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Full-length recombinant protein corresponding to aa1-253 from SPINT2 (AAH11951) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MAQLCGLRRSRAFLALLGSLLLSGVLAADRERSIHDFCLVSKVVGRCRASMPRWWYNVTDGSCQLFVYGGCDGNSNNYLTKEECLKKCATVTENATGDLATSRNAADSSVPSAPRRQDPEDHSSDMFNYEEYCTANAVTGPCRASFPRWYFDVERNSCNNFIYGGCRGNKNSYRSEEACMLRCFRQQENPPLPLGSKVVLLAGLFVMVSILFLGASMVYLIRVARRNQERALRTVWSSGDDKEQLVKNTYVL*
Conjugate
Biotin
Applications Tested/Suitable for anti-SPINT2 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-SPINT2 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-SPINT2 antibody

Western Blot analysis of SPINT2 expression in transfected 293T cell line by SPINT2 monoclonal antibody Lane 1: SPINT2 transfected lysate (Predicted MW: 28.2kD). Lane 2: Non-transfected lysate.
Mouse Anti-SPINT2 Monoclonal Antibody – Cat# MBS6144560

Testing Data of anti-SPINT2 antibody

Detection limit for recombinant GST tagged SPINT2 is 1ng/ml as a capture antibody.
Mouse Anti-SPINT2 Monoclonal Antibody, Testing Data – Cat# MBS6144560

Other Notes

Small volumes of anti-SPINT2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SPINT2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Related Accession #
NCBI Official Full Name
Homo sapiens serine peptidase inhibitor, Kunitz type, 2, mRNA
NCBI Official Synonym Full Names
serine peptidase inhibitor, Kunitz type 2
NCBI Official Symbol
SPINT2
NCBI Official Synonym Symbols
PB; Kop; HAI2; DIAR3; HAI-2
NCBI Protein Information
kunitz-type protease inhibitor 2

NCBI Summary for SPINT2

This gene encodes a transmembrane protein with two extracellular Kunitz domains that inhibits a variety of serine proteases. The protein inhibits HGF activator which prevents the formation of active hepatocyte growth factor. This gene is a putative tumor suppressor, and mutations in this gene result in congenital sodium diarrhea. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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