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MAK Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6148097

MBS6148097 is an antibody targeting MAK. Also known as MAK (Serine/Threonine-protein Kinase MAK, Male Germ Cell-associated Kinase, dJ417M14.2) (FITC). Reactive in Human. Host: Mouse. More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MAK, Monoclonal Antibody
Full Product Name
MAK (Serine/Threonine-protein Kinase MAK, Male Germ Cell-associated Kinase, dJ417M14.2) (FITC)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
4E9
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human MAK.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
66,345 Da
Immunogen
Partial recombinant corresponding to aa358-457 from human MAK (AAH39825) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
PPKQQSQEKPPQTLFPSIVKNMPTKPNGTLSHKSGRRRWGQTIFKSGDSWEELEDYDFGASHSKKPSMGVFKEKRKKDSPFRQQVKMAVISLSAHQFPTL
Conjugate
FITC

Product Categories/Family for anti-MAK antibody

Applications Tested/Suitable for anti-MAK antibody
ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)
Application Notes for anti-MAK antibody
IF: 10ug/ml
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-MAK antibody

Western Blot detection against Immunogen (36.41kD).
Mouse Anti-MAK Monoclonal Antibody – Cat# MBS6148097

Western Blot (WB) of anti-MAK antibody

MAK monoclonal antibody Western Blot analysis of MAK expression in Hela NE.
Mouse Anti-MAK Monoclonal Antibody, Western Blot (WB) – Cat# MBS6148097

Western Blot (WB) of anti-MAK antibody

Western Blot analysis of MAK expression in transfected 293T cell line by MAK monoclonal antibody. Lane 1: MAK transfected lysate (52.6kD). Lane 2: Non-transfected lysate.
Mouse Anti-MAK Monoclonal Antibody, Western Blot (WB) – Cat# MBS6148097

Immunofluorescence (IF) of anti-MAK antibody

Immunofluorescence of monoclonal antibody to MAK on HeLa cell. [antibody concentration 10ug/ml]
Mouse Anti-MAK Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6148097

Testing Data of anti-MAK antibody

Detection limit for recombinant GST tagged MAK is ~0.3ng/ml as a capture antibody.
Mouse Anti-MAK Monoclonal Antibody, Testing Data – Cat# MBS6148097

Other Notes

Small volumes of anti-MAK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MAK. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Homo sapiens male germ cell-associated kinase, mRNA
NCBI Official Synonym Full Names
male germ cell associated kinase
NCBI Official Symbol
MAK
NCBI Official Synonym Symbols
RP62
Protein Family
NCBI Protein Information
serine/threonine-protein kinase MAK

NCBI Summary for MAK

The product of this gene is a serine/threonine protein kinase related to kinases involved in cell cycle regulation. Studies of the mouse and rat homologs have localized the kinase to the chromosomes during meiosis in spermatogenesis, specifically to the synaptonemal complex that exists while homologous chromosomes are paired. Mutations in this gene have been associated with ciliary defects resulting in retinitis pigmentosa 62. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-MAK antibody

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