Rabbit Anti-Protein Synthesis Initiation Factor 4e Polyclonal Antibody – Cat# MBS615503

Western blot analysis of extracts from control and serum treated NIH/3T3, 293 or PC12 cells, using MBS615503.

Protein Synthesis Initiation Factor 4e Antibody – Rabbit Polyclonal

Name: Protein Synthesis Initiation Factor 4e Antibody; Polyclonal; Rabbit; MyBioSource
Brand: MyBioSource
SKU: MBS615503
Price: 755 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS615503

Eukaryotic initiation factor 4E (eIF4E) binds to the mRNA cap structure thereby mediating the initiation of translation (1,2). eIF4E interacts with eIF4G, which serves as a scaffold protein for the assembly of eIF4E and eIF4A to form the eIF4F complex (2).… More details

$755 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Protein Synthesis Initiation Factor 4E, Polyclonal Antibody

Full Product Name

Protein Synthesis Initiation Factor 4E (eIF 4E, Eukaryotic Translation Initiation Factor)

Product Synonym Names

Anti -Protein Synthesis Initiation Factor 4E (eIF 4E, Eukaryotic Translation Initiation Factor)

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human, Monkey, Mouse, Rat

Form/Format

Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

Purity/Purification

Affinity Purified
Purified by Protein A affinity chromatography.

Specificity

Recognizes endogenous levels of total human eIF4E protein. The antibody does not cross-react with other proteins. Species Crossreactivity: mouse, rat, monkey and zebra fish.

Preparation and Storage

May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunogen

Synthetic peptide corresponding to residues around Ser209 of eIF4E (KLH).

Product Categories/Family for anti-Protein Synthesis Initiation Factor 4E antibody

Antibodies; Abs to Translation Initiation Factors

Applications Tested/Suitable for anti-Protein Synthesis Initiation Factor 4E antibody

Western Blot (WB), Immunohistochemistry (IHC)

Application Notes for anti-Protein Synthesis Initiation Factor 4E antibody

Suitable for use in ELISA, Western Blot and Immunohistchemistry.
Dilution: Western Blot: 1:1000
Immunohistochemistry (Paraffin and Frozen): 1:100

Other Notes

Small volumes of anti-Protein Synthesis Initiation Factor 4E antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Protein Synthesis Initiation Factor 4E purchase

MBS615503 is an antibody targeting Protein Synthesis Initiation Factor 4E. Also known as Protein Synthesis Initiation Factor 4E (eIF 4E, Eukaryotic Translation Initiation Factor). Reactive in Human, Monkey, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Protein Synthesis Initiation Factor 4E. It may not necessarily be applicable to this product.

NCBI GI #

171446

NCBI Accession #

AAA34587.1

NCBI Official Full Name

protein synthesis initiation factor eIF-4E

UniProt Comments for Protein Synthesis Initiation Factor 4E

Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.