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TOLLIP Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6155477

MBS6155477 is an antibody targeting TOLLIP. Also known as TOLLIP (Toll Interacting Protein, FLJ33531, IL-1RAcPIP) (HRP). Reactive in Human. Host: Mouse. More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
TOLLIP, Monoclonal Antibody
Full Product Name
TOLLIP (Toll Interacting Protein, FLJ33531, IL-1RAcPIP) (HRP)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
1A5-2A3
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human TOLLIP.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
22,812 Da
Immunogen
Full length recombinant corresponding to aa1-275 from human TOLLIP (AAH04420) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MATTVSTQRGPVYIGELPQDFLRITPTQQQRQVQLDAQAAQQLQYGGAVGTVGRLNITVVQAKLAKNYGMTRMDPYCRLRLGYAVYETPTAHNGAKNPRWNKVIHCTVPPGVDSFYLEIFDERAFSMDDRIAWTHITIPESLRQGKVEDKWYSLSGRQGDDKEGMINLVMSYALLPAAMVMPPQPVVLMPTVYQQGVGYVPITGMPAVCSPGMVPVALPPAAVNAQPRCSEEDLKAIQDMFPNMDQEVIRSVLEAQRGNKDAAINSLLQMGEEP
Conjugate
HRP
Applications Tested/Suitable for anti-TOLLIP antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-TOLLIP antibody
ELISA: 1ng/ml
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-TOLLIP antibody

Western Blot detection against Immunogen (56.25kD).
Mouse Anti-TOLLIP Monoclonal Antibody – Cat# MBS6155477

Western Blot (WB) of anti-TOLLIP antibody

TOLLIP monoclonal antibody, Western Blot analysis of TOLLIP expression in 293.
Mouse Anti-TOLLIP Monoclonal Antibody, Western Blot (WB) – Cat# MBS6155477

Western Blot (WB) of anti-TOLLIP antibody

Western Blot analysis of TOLLIP expression in transfected 293T cell line by TOLLIP monoclonal antibody. Lane 1: TOLLIP transfected lysate (30.3kD). Lane 2: Non-transfected lysate.
Mouse Anti-TOLLIP Monoclonal Antibody, Western Blot (WB) – Cat# MBS6155477

Immunoprecipitation (IP) of anti-TOLLIP antibody

Immunoprecipitation of TOLLIP transfected lysate using TOLLIP monoclonal antibody and Protein A Magnetic Bead and immunoblotted with TOLLIP monoclonal antibody.
Mouse Anti-TOLLIP Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS6155477

Testing Data of anti-TOLLIP antibody

Detection limit for recombinant GST tagged TOLLIP is ~1ng/ml as a capture antibody.
Mouse Anti-TOLLIP Monoclonal Antibody, Testing Data – Cat# MBS6155477

Other Notes

Small volumes of anti-TOLLIP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TOLLIP. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Homo sapiens toll interacting protein, mRNA
NCBI Official Synonym Full Names
toll interacting protein
NCBI Official Symbol
TOLLIP
NCBI Official Synonym Symbols
IL-1RAcPIP
NCBI Protein Information
toll-interacting protein

NCBI Summary for TOLLIP

This gene encodes a ubiquitin-binding protein that interacts with several Toll-like receptor (TLR) signaling cascade components. The encoded protein regulates inflammatory signaling and is involved in interleukin-1 receptor trafficking and in the turnover of IL1R-associated kinase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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