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AURKB Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6166231

Chromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. The Aurora kinases associate with microtubules during chromosome movement and segregation. Aurora kinase B localizes to microtubules near kinetochores,… More details

$1140 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
AURKB, Monoclonal Antibody
Full Product Name
AURKB (Aurora Kinase B, AIK2, AIM-1, AIM1, ARK2, AurB, IPL1, STK12, STK5) (APC)
Product Gene Name
Product Synonym Names
Aurora Kinase B; AIK2; AIM-1; AIM1; ARK2; AurB; IPL1; STK12; STK5
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
6H7
Host
Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Purity/Purification
Purified
Specificity
Recognizes AURKB.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Immunogen
AURKB (AAH09751, 1aa-90aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MAQKENSYPWPYGRQTAPSGLSTLPQRVLRKEPVTPSALVLMSRSNVQPTAAPGQKVMENSSGTPDILTRHFTIDDFEIGRPLGKGKFGN
Conjugate
APC

Product Categories/Family for anti-AURKB antibody

Applications Tested/Suitable for anti-AURKB antibody
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-AURKB antibody
Applications are based on unconjugated antibody.

Testing Data of anti-AURKB antibody

Detection limit for recombinant GST tagged AURKB is approximately 0.03ng/ml as a capture antibody.
Mouse Anti-AURKB Monoclonal Antibody – Cat# MBS6166231

Immunofluorescence (IF) of anti-AURKB antibody

Immunofluorescence of monoclonal antibody to AURKB on HeLa cell. [antibody concentration 10 ug/ml]
Mouse Anti-AURKB Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6166231

Immunofluorescence (IF) of anti-AURKB antibody

Immunofluorescence of monoclonal antibody to AURKB on HeLa cell. [antibody concentration 10 ug/ml]
Mouse Anti-AURKB Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS6166231

Immunoprecipitation (IP) of anti-AURKB antibody

Immunoprecipitation of AURKB transfected lysate using anti-AURKB monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with AURKB monoclonal antibody.
Mouse Anti-AURKB Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS6166231

Western Blot (WB) of anti-AURKB antibody

Western Blot analysis of AURKB expression in transfected 293T cell line by AURKB monoclonal antibody (M03), clone 6H7.

Lane 1: AURKB transfected lysate (Predicted MW: 39.3 KDa).
Lane 2: Non-transfected lysate.
Mouse Anti-AURKB Monoclonal Antibody, Western Blot (WB) – Cat# MBS6166231

Other Notes

Small volumes of anti-AURKB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for AURKB purchase

MBS6166231 is an antibody targeting AURKB. Also known as AURKB (Aurora Kinase B, AIK2, AIM-1, AIM1, ARK2, AurB, IPL1, STK12, STK5) (APC). Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for AURKB. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Related Accession #
NCBI Official Full Name
Aurora kinase B
NCBI Official Synonym Full Names
aurora kinase B
NCBI Official Symbol
AURKB
NCBI Official Synonym Symbols
AIK2; AIM1; ARK2; AurB; IPL1; STK5; AIM-1; ARK-2; STK-1; STK12; PPP1R48; aurkb-sv1; aurkb-sv2
Protein Family
NCBI Protein Information
aurora kinase B

NCBI Summary for AURKB

This gene encodes a member of the aurora kinase subfamily of serine/threonine kinases. The genes encoding the other two members of this subfamily are located on chromosomes 19 and 20. These kinases participate in the regulation of alignment and segregation of chromosomes during mitosis and meiosis through association with microtubules. A pseudogene of this gene is located on chromosome 8. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2015]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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